FXR1 Rabbit pAb - P51114

Core Product Specifications and Parameters

Biological Background: FXR1 Function and Localization

• FXR1 (RNA-binding protein FXR1, FMR1 autosomal homolog 1) is an mRNA-binding protein that binds AU-rich elements (AREs) in the 3'-UTR of target mRNAs, regulating their translation and stability. It is required for neurogenesis, muscle development, and spermatogenesis (PubMed:17382880, PubMed:20417602, PubMed:30067974). Related references: PMID:17382880 | PMID:20417602 | PMID:30067974

• In muscle tissue, FXR1 promotes assembly of target mRNAs into cytoplasmic ribonucleoprotein granules via liquid-liquid phase separation, essential for embryonic and postnatal muscle development (PubMed:30770808). Related references: PMID:30770808

• FXR1 promotes translation of MYC transcripts by recruiting the eIF4F complex to the translation start site (PubMed:34731628). Related references: PMID:34731628

• Acts as a negative regulator of inflammation by destabilizing pro-inflammatory transcripts in response to IL19 (PubMed:30067974). Related references: PMID:30067974

• Involved in nuclear pore complex localization by preventing cytoplasmic aggregation of nucleoporins via a microtubule-dependent mechanism (PubMed:32706158). Related references: PMID:32706158

• Forms a cytoplasmic messenger ribonucleoprotein (mRNP) network by packaging long mRNAs, serving as a scaffold that recruits signaling molecules, crucial for actomyosin reorganization (PubMed:39106863). Related references: PMID:39106863

• Subcellular localization includes cytoplasm, stress granules, dendrites, axons, nuclear envelope, and postsynaptic sites.

• Expressed in all tissues examined, with high levels in brain neurons, especially in Purkinje cells (PubMed:7781595, PubMed:9259278). Related references: PMID:7781595 | PMID:9259278

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 310–539, a region conserved across human, mouse, and rat FXR1; this polyclonal antibody may recognize multiple isoforms due to alternative splicing.
• In Western blotting, expect a band around 70 kDa; additional bands may appear from splice variants or post-translational modifications.
• For immunofluorescence/ICC, permeabilization is required to access cytoplasmic and granule-localized FXR1; co-staining with organelle markers may help discern specific localization.
• Pre-adsorption with the immunogen can serve as a negative control; validate specificity using siRNA knockdown or CRISPR knockout models in relevant cell lines.
• ELISA assays may require optimization of coating antigen and antibody concentration; consider using recombinant FXR1 protein as standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your strategic sourcing partner for IVD raw materials, offering validated antibodies like this FXR1 polyclonal antibody for research in mRNA regulation, neurodevelopment, and muscle biology. Beyond this product, we supply optimized antibody pairs, bulk monoclonal and polyclonal reagents, and ancillary raw materials to accelerate your diagnostic development. Let CamelBio bridge the gap from concept to clinic with reliable, one-stop access to rare target reagents and technical support.