FUT3 Rabbit Polyclonal Antibody (FucT-III) for WB and ELISA - P21217

Core Product Specifications and Parameters

Biological Background: FUT3 Function and Localization

• FUT3 (Fucosyltransferase 3, also known as FucT-III, CD174, Lewis FT) is a Golgi membrane-bound glycosyltransferase responsible for the synthesis of Lewis blood group antigens, including Lewis a, Lewis b, Lewis x (SSEA-1), and Lewis y, through the transfer of L-fucose to type 1 and type 2 oligosaccharide acceptors. Related references: PMID:11058871, PMID:12668675, PMID:1977660

• It catalyzes alpha(1,4)-fucosylation of type 1 chains (Galβ1-3GlcNAc) and alpha(1,3)-fucosylation of type 2 chains (Galβ1-4GlcNAc), with a preference for type 1 acceptors, and can also utilize sialylated precursors to generate sialyl Lewis a (sLe^a) and sialyl Lewis x (sLe^x) epitopes. Related references: PMID:11058871, PMID:12668675, PMID:7721776

• The enzyme plays a critical role in cell adhesion, migration, and proliferation by regulating the expression of sLe^a and sLe^x on the cell surface, which are ligands for E-selectin. Related references: PMID:11058871, PMID:27453266, PMID:29593094

• FUT3 is highly expressed in epithelial tissues of the stomach, colon, small intestine, lung, and kidney, with lower expression in salivary gland, bladder, uterus, and liver.

• The protein is anchored to the Golgi stack membrane, typical of glycosyltransferases, and its activity defines the Lewis blood group phenotype: Lewis-positive individuals have active FUT3, while Lewis-negative individuals carry inactivating mutations. Related references: PMID:1977660

• Keywords: Blood group antigen, Glycosyltransferase, Golgi apparatus, Lipid metabolism, Transmembrane, Signal-anchor.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1–100) of human FUT3; therefore, the antibody may recognize both native and denatured forms. For Western blot, a band at approximately 42 kDa is expected. Consider using standard reducing conditions and a suitable blocking agent.
• For ELISA, validate the antibody with the immunogen peptide or recombinant FUT3 protein to confirm reactivity. Pair with an appropriate secondary antibody for detection.
• Cross-reactivity with rat is reported. Validate in the relevant sample system and consider sequence alignment to assess potential recognition of other FUT family members.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutions with streamlined access to IVD raw materials. This anti-FUT3 polyclonal antibody is well-suited for investigations into Lewis antigen biosynthesis and glycosyltransferase-related disorders. As your sourcing partner, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement—covering every stage from concept to clinic.