FUK Rabbit Polyclonal Antibody for WB and ELISA - Q8N0W3

Core Product Specifications and Parameters

Biological Background: FUK Function and Localization

• FUK (fucokinase) is the enzyme L-fucose kinase, which catalyzes the phosphorylation of L-fucose, the initial step in the salvage pathway for recycling fucose from degraded oligosaccharides.
• The protein contains ATP-binding and nucleotide-binding sites, functioning as a kinase and transferase, essential for its enzymatic activity.
• Encoded by the FCSK gene, FUK undergoes alternative splicing, generating multiple isoforms that may have distinct roles.
• Tissue expression is observed in fibroblasts, suggesting a role in extracellular matrix or cell-surface fucosylation processes.
• Genetic variations in FCSK are linked to congenital disorders of glycosylation, underscoring the protein's importance in normal glycoprotein synthesis.
• The salvage pathway mediated by FUK contributes to the pool of GDP-fucose, a critical donor substrate for fucosyltransferases involved in diverse biological functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 795–1084 within the C‑terminal region of FUK; verify detection of full‑length and potential isoforms in relevant cell or tissue lysates.
• For Western blotting, the predicted molecular weight is 118 kDa; use an appropriate gel percentage (e.g., 8–10% acrylamide) for efficient transfer of high‑molecular‑weight proteins.
• Validate antibody specificity in your system by including positive controls (e.g., fibroblast lysates) and negative controls (e.g., FCSK‑knockout samples) where available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, from validated antibody pairs to bulk ancillary reagents. For targets like FUK, involved in glycosylation pathways, we offer polyclonal and monoclonal antibodies, recombinant proteins, and assay development support to accelerate your project from concept to clinic. Our sourcing expertise includes rare‑target reagents, enabling seamless integration into your diagnostic workflows.