FUCA2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9BTY2

Core Product Specifications and Parameters

Biological Background: FUCA2 Function and Localization

• FUCA2 encodes plasma alpha-L-fucosidase, also known as alpha-L-fucoside fucohydrolase 2 or alpha-L-fucosidase 2, a secreted enzyme involved in glycoprotein metabolism.
• The enzyme specifically hydrolyzes the alpha-1,6-linked fucose attached to the reducing-end N-acetylglucosamine of carbohydrate moieties on glycoproteins.
• As a member of the glycosyl hydrolase family, FUCA2 functions as a glycosidase and hydrolase, playing a key role in the degradation of fucosylated glycans.
• Subcellular localization studies indicate that FUCA2 is secreted and acts in the extracellular space, consistent with its role in clearing plasma glycoproteins.
• The protein is post‑translationally modified by glycosylation and phosphorylation, as reflected in its UniProt keywords “Glycoprotein” and “Phosphoprotein.”
• Alternative splicing of the FUCA2 transcript generates multiple isoforms, adding functional diversity.
• The predicted molecular weight of the full‑length protein is approximately 54 kDa (UniProt: 54067 Da), matching the observed band in Western blot.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a large internal fragment (aa 118‑467) of human FUCA2, likely recognizing both endogenous and recombinant forms of the enzyme.
• For Western blot, the expected band is around 54 kDa; use appropriate positive controls (e.g., human, mouse, or rat tissue lysates known to express FUCA2) and optimize lysate preparation to preserve the secreted protein.
• In IHC‑P, consider heat‑induced antigen retrieval and validate staining with tissue sections known to contain plasma‑derived or locally secreted FUCA2.
• ELISA performance may depend on the coating antigen and detection format; it is advisable to titrate the antibody and confirm specificity using negative controls.
• Cross‑reactivity with mouse and rat has been reported; however, optimal working dilutions for each species and application should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD raw‑material procurement by offering validated antibody pairs, bulk ancillary reagents, and access to rare‑target antibodies such as this anti‑FUCA2 polyclonal antibody. Whether you are developing diagnostic assays for glycoprotein disorders or conducting glycobiology research, our integrated supply chain and technical support help accelerate your project from concept to clinic. Count on us as a single, reliable partner for monoclonal, polyclonal, and recombinant antibody sourcing tailored to your diagnostic manufacturing needs.