FTMT Rabbit Polyclonal Antibody (Mitochondrial Ferritin) for WB, ELISA - Q8N4E7

Core Product Specifications and Parameters

Biological Background: FTMT Function and Localization

• FTMT encodes mitochondrial ferritin, a member of the ferritin family that stores iron in a bioavailable and non-toxic form.
• Catalyzes the ferroxidase reaction converting Fe2+ to Fe3+, enabling iron deposition as ferric hydroxides; essential for mitochondrial iron homeostasis. Related references: PMID:11323407, PMID:15201052
• Localized exclusively to the mitochondrion, playing a key role in mitochondrial iron metabolism and protection against oxidative stress.
• Tissue expression: predominantly detected in testis and erythroleukemia; very low or absent in brain, colon, heart, kidney, liver, lung, muscle, placenta, spleen, and small intestine.
• Contains a mitochondrial transit peptide that directs its import into the organelle.
• Associated with keywords: Iron storage, metal-binding, oxidoreductase; structural studies available (3D-structure).

Experimental Guidance and Technical Tips

• For WB, expected molecular weight is ~28 kDa. Optimize blocking and detection conditions to minimize background from polyclonal antibodies.
• The immunogen corresponds to C-terminal region (aa 183-242) of human FTMT; verify target specificity using appropriate controls, such as knockout cell lysates or peptide competition.
• Cross-reactivity with mouse FTMT allows potential use in mouse tissue or cell models; validate in your specific sample matrix.
• For ELISA, use the recombinant immunogen or purified protein as standard; assess antibody titer and specificity by indirect ELISA.
• Consider including a mitochondrial enrichment step or subcellular fractionation to enhance detection in low-expressing tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to high-quality IVD raw materials, supporting every stage from concept to clinic. Whether you need validated antibody pairs for mitochondrial biomarkers, optimized polyclonal antibodies like this anti-FTMT, or bulk ancillary reagents, our team ensures reliable supply and technical support. For rare targets like mitochondrial ferritin, we offer custom sourcing and assay development assistance to accelerate your diagnostic or research projects.