FTCD Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - O95954

Core Product Specifications and Parameters

Biological Background: FTCD Function and Localization

• FTCD, also known as formiminotransferase-cyclodeaminase or LCHC1, is a bifunctional enzyme essential for folate-dependent one-carbon metabolism.
• It channels one-carbon units from formiminoglutamate to the folate pool via its transferase and deaminase activities.
• FTCD also binds and promotes bundling of vimentin filaments originating from the Golgi apparatus, linking metabolism to cytoskeletal dynamics.
• The protein is primarily cytoplasmic, with specific localization in the cytosol, Golgi apparatus, centrosomes, and centrioles.
• As a multifunctional enzyme, FTCD exhibits lyase and transferase activities and is involved in histidine metabolism.
• Alternative splicing of the FTCD gene gives rise to multiple transcript variants, potentially increasing functional diversity.
• The protein is subject to phosphorylation, suggesting post-translational regulation of its dual functions.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 320-541) targets a central region of human FTCD; consider it for detecting all known isoforms in relevant sample types.
• For Western blotting (predicted MW: 59 kDa), validate the antibody in lysates from human, mouse, or rat tissues/cells expressing FTCD.
• Immunofluorescence and immunohistochemistry (IF-P, IHC-P) should reveal cytosolic and Golgi staining; optimize fixation and permeabilization to preserve antigenicity.
• In ELISA applications, this monoclonal antibody can serve as a capture or detection reagent; pairing with a different clone is recommended for sandwich assays.
• Although the antibody is raised against human FTCD, cross-reactivity with mouse and rat orthologs has been observed—verify sequence homology before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides this FTCD Rabbit Monoclonal Antibody as part of its extensive portfolio of IVD raw materials, supporting research into folate metabolism and cytoskeleton biology. Whether you need validated antibody pairs, bulk monoclonal/polyclonal reagents, or ancillary raw materials, our sourcing platform streamlines procurement from discovery through clinical stages. For rare or custom targets, CamelBio offers specialized sourcing to help bridge your supply gaps with reliable quality.