FSHB Rabbit Monoclonal Antibody for IF-P, IHC-P, ELISA - P01225

Core Product Specifications and Parameters

Biological Background: Follitropin Subunit Beta Function and Localization

• FSHB encodes the beta subunit of follicle-stimulating hormone (FSH), which dimerizes with the alpha chain CGA to form the biologically active glycoprotein hormone.
• The FSH heterodimer binds to the FSHR receptor, a G protein-coupled receptor, activating downstream signaling pathways essential for reproductive function.
  Related references: PMID:24692546 PMID:2494176
• Follitropin plays an essential role in follicle development and spermatogenesis.
  Related references: PMID:407105 PMID:8220432
• The protein is secreted and possesses disulfide bonds and N-linked glycosylation, characteristic of the glycoprotein hormone family.
• Mutations in FSHB are associated with hypogonadotropic hypogonadism, leading to delayed or absent puberty and infertility.
• FSH pharmaceutical formulations are used clinically to treat fertility disorders, highlighting its therapeutic importance.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 25-116 of human FSH alpha/beta, covering the mature region; the antibody may recognize the native secreted form of FSHB.
• For IHC-P and IF-P, standard antigen retrieval methods (e.g., heat-induced epitope retrieval at pH 6.0 or 9.0) are suggested; optimize fixation conditions based on sample type.
• Validate the antibody cross-reactivity in mouse and rat tissues, as predicted; species-specific epitope accessibility should be confirmed in pilot studies.
• For ELISA, pair with a suitable detection antibody recognizing a distinct epitope; check dilution linearity and matrix effects using recombinant FSHB or native sources.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive supply chain for IVD raw materials, including validated monoclonal antibodies like this anti-FSHB clone. We support diagnostic manufacturers and research laboratories with bulk antibody sourcing, optimized antibody pairs, and ancillary reagents. Whether your work focuses on reproductive hormone assays or fertility diagnostics, CamelBio streamlines access to critical tools from concept to clinic.