FOXP3 Rabbit Monoclonal Antibody for IHC-P and ELISA - Q9BZS1

Core Product Specifications and Parameters

Biological Background: FOXP3 Function and Localization

• FOXP3, also known as Scurfin, is a forkhead box transcription factor essential for the development and suppressive function of regulatory T-cells (Treg). It maintains immune homeostasis by ensuring Treg lineage stability and modulating conventional T-cell expansion. Related references: PMID:17377532, PMID:21458306, PMID:23947341, PMID:23169781

• FOXP3 can act as either a transcriptional repressor or activator depending on interactions with co-factors. It activates genes like CTLA4 and TNFRSF18 while repressing IL2 and IFNG, thereby directly modulating immune responses. Related references: PMID:17377532, PMID:21458306, PMID:23947341

• FOXP3 inhibits cytokine production and T-cell effector function by repressing key transcription factors RELA and NFATC2. Related references: PMID:15790681

• Transcriptional repression of IL2 by FOXP3 involves association with histone acetylase KAT5 and histone deacetylase HDAC7. Related references: PMID:17360565

• FOXP3 cooperates with transcription factor RUNX1 to regulate expression of targets such as TNFRSF18, IL2RA, CTLA4, IL2, and IFNG. Related references: PMID:17377532

• FOXP3 inhibits differentiation of Th17 cells by antagonizing RORC and RORA, down-regulating IL17 expression and favoring Treg development. Related references: PMID:18368049, PMID:18354202

• FOXP3 localizes to both the nucleus and cytoplasm and is subject to post-translational modifications including acetylation, phosphorylation, and ubiquitination. It contains a zinc-finger domain critical for DNA binding and is associated with the immune disorder IPEX.

Experimental Guidance and Technical Tips

• For IHC-P, consider using formalin-fixed paraffin-embedded tissue sections with appropriate antigen retrieval methods. Optimization of detection conditions may be required for robust staining.
• The antibody reacts with human and mouse FOXP3; validate cross-reactivity in your specific experimental model to ensure specificity.
• Being a rabbit monoclonal antibody, it is expected to offer high affinity and lot-to-lot consistency, but individual assay validation is recommended.
• For ELISA, pairing with a suitable detection antibody and proper coating antigen (e.g., recombinant FOXP3 or peptide) is critical. Include appropriate positive and negative controls.
• Store and handle according to shipping conditions (ice bag upon receipt), with aliquoting to minimize freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutions with one-stop access to IVD raw materials, technical services, and consulting, supporting every stage from concept to clinic. For immunology and Treg research, we supply validated antibody pairs, optimized monoclonal antibodies like FOXP3 Rabbit mAb, and bulk ancillary reagents, ensuring reliable sourcing for your assay development needs.