FOXP1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9H334

Core Product Specifications and Parameters

Biological Background: FOXP1 Function and Localization

• FOXP1 is a transcriptional repressor that can synergize with CTBP1 to repress transcription, though CTBP1 is not essential. Related references: PMID:18347093 PMID:26647308
• It plays a critical role in lung epithelium specification and differentiation, cooperating with FOXP4 to regulate secretory cell fate by restricting goblet cell lineage.
• Acts as an essential transcriptional regulator of B-cell development and can repress pro-apoptotic genes to promote B-cell expansion via NF-kappa-B signaling. Related references: PMID:25267198
• Negatively regulates the differentiation of T follicular helper cells and is involved in monocyte/macrophage differentiation by binding to the CSF1R promoter. Related references: PMID:15286807 PMID:18347093 PMID:18799727
• Involved in spinal motor neuron organization, promoting formation of lateral motor neuron column and preganglionic motor column.
• Participates in cardiac muscle cell proliferation regulation and angiogenesis, where it suppresses SEMA5B to promote endothelial cell functions. Related references: PMID:24023716
• Isoform 8 is specifically expressed in embryonic stem cells and stimulates pluripotency gene expression while repressing differentiation genes. Related references: PMID:21924763
• Localized to the nucleus, FOXP1 contains zinc-finger domains and undergoes post-translational modifications including phosphorylation and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen (aa 550-677) includes part of the forkhead DNA-binding domain, which is highly conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blotting, expect a band around 75 kDa; note that isoform 8 (embryonic stem cell-specific) may show a different size.
• Validate antibody specificity in your experimental system, as FOXP1 has multiple isoforms and tissue-specific expression.
• For immunohistochemistry (paraffin), optimize antigen retrieval; nuclear staining is anticipated.
• For ELISA, perform titration and include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing platform for IVD raw materials, bridging diagnostic manufacturers and researchers with products like this FOXP1 polyclonal antibody. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. Our supply chain supports transcription factor research, immunology, and stem cell applications from concept to clinical use.