FOXO4 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P98177

Core Product Specifications and Parameters

Biological Background: FOXO4 Function and Localization

• FOXO4 (Forkhead box protein O4), also known as AFX1, is a member of the forkhead family of transcription factors characterized by a distinct DNA-binding forkhead domain.
• It functions as a key regulator of the insulin signaling pathway by binding to insulin-response elements (IREs) and activating transcription of target genes such as IGFBP1.
• FOXO4 negatively regulates the cell cycle and can suppress hypoxia-induced transcriptional activation by down-regulating HIF1A expression.
• In embryonic stem cells, FOXO4 enhances proteasome activity by upregulating PSMD11 expression, promoting 26S proteasome assembly.
• The protein shuttles between the cytoplasm and nucleus, with its subcellular localization regulated by post-translational modifications including phosphorylation and acetylation.
• FOXO4 is widely expressed across tissues, with highest levels in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas; isoform zeta is predominant in liver, kidney, and pancreas.
• It has been implicated in diverse processes such as cellular differentiation, myogenesis, and oncogenesis, with involvement in chromosomal rearrangements linked to leukemias.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 230-341) of human FOXO4, which lies outside the conserved forkhead domain; this design may help in detecting specific isoforms or modifications in this region.
• For Western blot (WB), a specific band around 54 kDa is expected; validate in lysates from human, mouse, or rat cell lines known to express FOXO4, such as HEK293, MCF-7, or H9c2.
• In immunofluorescence/immunocytochemistry (IF/ICC), consider the stimuli-dependent nuclear/cytoplasmic shuttling of FOXO4; optimize fixation and permeabilization conditions to preserve localization.
• For ELISA applications, use the antibody as a capture or detection reagent in combination with a matched pair to ensure assay sensitivity; titrate antibody concentrations using recombinant FOXO4 protein as standard.
• Given the broad tissue expression, include appropriate tissue controls and consider the presence of alternative isoforms that may influence band patterns in WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for researchers and diagnostic manufacturers by offering a curated portfolio of antibodies, including this FOXO4 polyclonal antibody for transcription and cell cycle studies. Beyond individual reagents, we supply validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary critical raw materials. For rare or custom targets like FOXO4 pathway components, our sourcing expertise ensures reliable, scalable supply to accelerate your assay development from concept to clinic.