FOXL2 Rabbit pAb for WB, IF-P, ELISA - P58012

Core Product Specifications and Parameters

Biological Background: FOXL2 Function and Localization

• FOXL2 is a forkhead box transcription factor critical for ovary differentiation and maintenance, and functions as a repressor of the somatic testis determination program.
• It prevents ovary-to-testis trans-differentiation through transcriptional repression of the Sertoli cell-promoting gene SOX9 (by similarity).
• FOXL2 has apoptotic activity in ovarian cells and modulates estrogen signaling by suppressing ESR1-mediated PTGS2/COX2 transcription and regulating CYP19 expression (by similarity).
• It participates in SMAD3-dependent transcription of FST and activates SIRT1 under cellular stress conditions, as well as OSR2 transcription.
• FOXL2 is localized exclusively in the nucleus.
• It is expressed early in the somatic cells of the developing gonad and persists in follicular cells of the adult ovary, in addition to developing eyelid.
• The protein is a phosphoprotein and is modified by ubiquitin conjugation (isopeptide bond); it is associated with premature ovarian failure and features a triplet repeat expansion.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning residues 300-376 within the C-terminus of human FOXL2; this region may be conserved in mouse and rat, supporting reported cross-reactivity.
• For Western blotting (WB), a band around 39 kDa is expected; optimal antibody dilution and blocking conditions should be empirically determined.
• In immunofluorescence (IF-P), since FOXL2 is a nuclear protein, appropriate nuclear counterstaining and permeabilization protocols are recommended.
• For ELISA applications, the antibody can be evaluated as a capture or detection reagent; consider pairing with another FOXL2-specific antibody for sandwich formats.
• Always validate the antibody in the relevant species and sample type, and include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibodies, optimized reagents, and technical support from concept to clinic. For those developing assays in reproductive endocrinology or ovarian biology, our FOXL2 Rabbit pAb and related portfolio can streamline your R&D and manufacturing. We also provide bulk ancillary reagents and rare target sourcing to meet your unique needs.