FOXF1 Rabbit Monoclonal Antibody for WB and ELISA - Q12946

Core Product Specifications and Parameters

Biological Background: FOXF1 Function and Localization

• FOXF1, also known as forkhead box protein F1, forkhead-related activator 1 (FREAC-1), and FKHL5, belongs to the forkhead family of transcription factors.
• It acts as a probable transcription activator for lung-specific genes, implying a critical role in pulmonary development and homeostasis.
• As a member of the forkhead box (FOX) family, it contains a conserved DNA-binding domain characteristic of the family.
• FOXF1 localizes predominantly to the nucleus, consistent with its function as a transcription regulator.
• Expression is detected in lung and placenta, indicating tissue-specific regulatory activities.
• Disease variants, such as those associated with alveolar capillary dysplasia with misalignment of pulmonary veins (ACDMPV), underscore its importance in lung morphogenesis.

Experimental Guidance and Technical Tips

• The immunogen targets the N-terminal region (amino acids 1-100) of human FOXF1; consider that this antibody may detect full-length protein but might not recognize isoforms lacking this region.
• For Western blot, the expected band is approximately 40 kDa; validate in nuclear extracts of relevant cell lines or tissues (e.g., lung, placenta) and optimize antibody dilution and blocking conditions.
• In ELISA, use as a capture or detection reagent; pairing with a second antibody against a different epitope can enhance assay specificity.
• Cross-reactivity is confirmed for human and mouse; if working with other species, validate recognition due to potential sequence homology.
• Use nuclear extraction protocols for optimal sample preparation, as FOXF1 is a nuclear protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your diagnostic development by providing ready-to-use research materials like this FOXF1 monoclonal antibody, validated for WB and ELISA. As your one-stop partner for IVD raw materials, we offer a comprehensive portfolio of antibodies, antigens, and reagents, along with technical support, to accelerate your projects from concept to clinic. Contact us for bulk orders, custom antibody development, and specialized sourcing needs in respiratory disease research.