Formyl-Histone H2B-K120 Rabbit Monoclonal Antibody (P62807/Q16778) for WB, IHC-P, ELISA

Core Product Specifications and Parameters

Biological Background: Formyl-Histone H2B Function and Localization

Histone H2B is a core component of the nucleosome, where it wraps and compacts DNA into chromatin, thus playing a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. The dynamic accessibility of DNA is governed by the "histone code," a complex set of post-translational modifications (PTMs) on histones. Related references: UniProt P62807, UniProt Q16778

Human Histone H2B undergoes extensive PTMs, including acetylation (e.g., at K6, K12, K16, K21), crotonylation (K12), phosphorylation (S15), and formylation at K120. Formylation at K120 is an emerging modification that may subtly influence chromatin architecture and gene expression, though its full biological significance remains under active investigation. Related references: PMID:16283522, PMID:16627869, PMID:21925322, PMID:12757711

In addition to its structural role, H2B exhibits broad antibacterial activity and may contribute to the antimicrobial barrier of the colonic epithelium and the bactericidal activity of amniotic fluid. Related references: UniProt P62807, UniProt Q16778

H2B localizes predominantly to the nucleus and associates with chromosomes. The human genome encodes multiple H2B variants, including H2B type 1-C/E/F/G/I (P62807) and H2B type 2-E (Q16778), which share highly conserved sequences. The immunogen peptide spans a region conserved across these isoforms, supporting the predicted wide cross-reactivity. Related references: UniProt P62807, UniProt Q16778

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to human Histone H2B with formylation at lysine 120. The antibody is expected to specifically recognize formyl-H2B-K120; consider using a non-formylated peptide or enzymatic de-formylation as a negative control to confirm specificity.
• For Western blotting (WB), a band at approximately 14 kDa is expected. Use nuclear extracts or histone-enriched preparations. Inclusion of a low-molecular-weight marker and a control sample (e.g., untreated vs. formylase inhibitor-treated) is recommended.
• For immunohistochemistry (IHC-P), antigen retrieval may be necessary to expose the nuclear epitope in formalin-fixed paraffin-embedded sections. Nuclear staining should be observed; validate with appropriate tissue controls.
• For ELISA, coat the formylated peptide and use an unmodified peptide as a control to assess binding specificity. Optimize coating conditions and antibody dilution based on the sample matrix.
• Due to high sequence identity, the antibody is predicted to cross-react with human, mouse, and rat H2B; however, validation in the relevant species and sample system is advised.
• Avoid non-specific blocking agents that could mask the small-molecule modification; BSA-free blockers or alternative blocking strategies may be considered if background is observed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with a one-stop sourcing solution for IVD raw materials, covering every stage from concept to clinical application. This formyl-Histone H2B-K120 rabbit monoclonal antibody (targeting P62807/Q16778) exemplifies our commitment to supplying high-quality, validated antibodies for epigenetic and chromatin research. Beyond individual reagents, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to accelerate your assay development and production.