FMN1 Rabbit pAb for WB, ELISA - Q68DA7

Core Product Specifications and Parameters

Biological Background: Formin-1 Function and Localization

• Formin-1 (FMN1), also known as limb deformity protein homolog, is an actin-binding protein involved in cytoskeletal organization.
• It plays a critical role in the formation of adherens junctions and the polymerization of linear actin cables, essential for cell adhesion and morphology.
• The protein is localized to multiple cellular compartments including the nucleus, cytoplasm, cell membrane, and specifically to adherens junctions at cell–cell contacts.
• FMN1 undergoes alternative splicing and contains coiled-coil domains, contributing to its structural versatility. Post-translational modifications include phosphorylation.
• Keywords associated with FMN1 highlight its functions: Actin-binding, Cell junction, Cell membrane, Coiled coil, and Phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to amino acids 350–495 of human FMN1. When designing detection systems, consider epitope accessibility within this region, especially in native protein conformations.
• This polyclonal antibody is suitable for Western blot (WB) and ELISA as specified. For WB, optimize loading amounts and blocking conditions; a band around 158 kDa (or the observed molecular weight of the target isoform) may be expected.
• Cross-reactivity with mouse has been reported. Validate antibody specificity in your model system using appropriate controls (e.g., knockout tissue or cell lines) to confirm target recognition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. For your FMN1/actin cytoskeleton research needs, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and can assist in sourcing rare-target raw materials to streamline your assay development.