FLII Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q13045

Core Product Specifications and Parameters

Biological Background: FLII (Flightless-1 Homolog) Function and Localization

• FLII, also known as flightless-1 homolog, is a member of the gelsolin family of actin-binding proteins and contains characteristic leucine-rich repeats (LRRs).
• It acts as a critical regulator of actin polymerization, essential for proper myofibril organization and the regulation of sarcomeric thin filament length in muscle cells.
• Plays an important role in the assembly of cardiomyocyte cell adhesion complexes, contributing to the structural integrity of cardiac muscle.
• Modulates cytoskeletal rearrangements during cytokinesis and cell migration by inhibiting Rac1-dependent paxillin phosphorylation.
• Functions as a transcriptional coactivator for hormone-activated nuclear receptors, cooperating with NCOA2 and CARM1, and participates in estrogen hormone signaling. Related references: PMID:14966289
• Subcellularly localizes to multiple compartments including the nucleus, cytoplasm, cytoskeleton, centrosomes, podosomes, and focal adhesions.
• Highly expressed in skeletal muscle, heart, and lung, with implications in muscle physiology and cardiomyopathy.
• Subject to post-translational modifications such as acetylation and phosphorylation; associated with disease variants and alternative splicing events.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 400–660 of human FLII; this central region may be conserved across species (confirmed reactivity with human, mouse, and rat), suggesting its utility in multi-species experiments.
• For Western blotting (WB), expect a band near 145 kDa; validate specificity in your sample system and consider optimizing blocking and detection conditions.
• In immunohistochemistry (IHC-P), pilot testing with appropriate positive (e.g., skeletal muscle) and negative controls is recommended to evaluate tissue staining patterns.
• ELISA protocols may be adapted for both direct and sandwich formats; preliminary checkerboard titrations are advised to determine optimal antibody and antigen concentrations.
• Given multiple isoforms and potential post-translational modifications, confirm the identity of detected bands using additional controls or orthogonal techniques.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw material supplier, CamelBio provides validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to support diagnostic and research workflows. This anti-FLII rabbit polyclonal antibody, targeting a key cytoskeletal and transcriptional regulator, is suited for studies in cell migration, muscle biology, and cardiac disease. CamelBio offers one-stop sourcing solutions for rare targets and large-scale production needs, helping you accelerate development from concept to clinic.