FITC Rabbit anti-Rat CD45 Monoclonal Antibody for FC - P04157

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45, also known as leukocyte common antigen (L-CA) and T200, is encoded by the PTPRC gene in rats. It is a receptor-type tyrosine-protein phosphatase essential for T-cell activation through the antigen receptor.
• The protein has two phosphatase domains; the first domain is catalytically active, while the second modulates substrate specificity. CD45 dephosphorylates key signaling molecules like SKAP1, FYN, and LYN upon T-cell activation.
• CD45 acts as a positive regulator of T-cell coactivation upon binding to DPP4, and it interacts with CLEC10A at the antigen-presenting cell–T-cell interface to modulate activation thresholds and limit autoreactivity.
• It is localized to the cell membrane, specifically enriched in membrane rafts and at the immunological synapse, where it participates in T-cell receptor signaling.
• Alternative splicing generates multiple isoforms; in rats, isoform 1 and isoform 2 are found in thymocytes and lymph nodes, while isoform 3 and isoform 4 are detected in lymph nodes.
• Post-translational modifications include heavy glycosylation and phosphorylation, which are critical for its function and interaction with ligands.
• The protein is categorized under hydrolases, protein phosphatases, and is a transmembrane glycoprotein involved in key immune processes.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 24–546 of rat CD45, which likely corresponds to the extracellular domain. This design typically generates antibodies that recognize cell surface-expressed CD45, well-suited for flow cytometry.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and staining conditions. Use appropriate isotype controls and single-stain compensation controls.
• Since CD45 is a pan-leukocyte marker, ensure that your sample preparation preserves leukocyte surface antigens; fresh or properly cryopreserved cells are recommended.
• Consider that CD45 isoforms vary among leukocyte subsets and activation states; validate the antibody’s staining pattern on your target populations to confirm reactivity with the relevant isoform(s).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including this anti-CD45 antibody for leukocyte analysis. From validated antibody pairs and bulk ancillary reagents to sourcing rare targets, we streamline your development pipeline from concept to clinic. Contact us to explore how our supply chain expertise can accelerate your next immunodiagnostic project.