FITC Rabbit Anti-Mouse CD32 Monoclonal Antibody for Flow Cytometry - P08101

Core Product Specifications and Parameters

Biological Background: Low Affinity Immunoglobulin Gamma Fc Region Receptor II (CD32) Function and Localization

• Encoded by the Fcgr2b gene, the Fc gamma receptor IIB (CD32) is a low‑affinity receptor for the Fc region of complexed IgG, existing as multiple isoforms (IIB1, IIB1', IIB2, and a secreted IIB3) generated by alternative splicing.
• Core functions include phagocytosis of immune complexes, modulation of antibody production by B‑cells, and down‑regulation of B‑cell, T‑cell, and mast cell activation upon co‑engagement with antigen receptors.
• Isoform IIB2 mediates endocytosis of soluble immune complexes via clathrin‑coated pits, while IIB1 and IIB1' form caps but do not internalize.
• The receptor is widely expressed on cells of hemopoietic origin, with IIB1 preferentially in lymphoid lineage, IIB2 in myeloid lineage, and IIB3 secreted by macrophages into serum.
• Subcellular localization includes the cell membrane, cytoplasmic/cytoskeletal compartments, and, for isoform IIB3, secretion.
• Post‑translational features include disulfide bonds, glycosylation, and phosphorylation (phosphoprotein), consistent with its immunoglobulin‑domain architecture.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (aa 30–207), suggesting the antibody likely recognizes the ligand‑binding domain common to major isoforms; however, isoform‑specific detection should be validated in the relevant sample system.
• For flow cytometry, titrate the FITC‑conjugated antibody on a known CD32‑expressing cell line (e.g., mouse splenocytes or a macrophage line) to determine optimal staining concentration and minimize non‑specific binding.
• Include appropriate isotype controls and Fc receptor blocking steps to ensure specificity when analyzing fresh hemopoietic cells.
• Consider that the observed molecular weight may vary (31–36 kDa) depending on glycosylation and isoform; confirm detection by Western blotting if cross‑application validation is needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a full spectrum of IVD raw materials, from validated antibody pairs and optimized monoclonals to bulk ancillary reagents and rare‑target sourcing. For projects involving immune receptor CD32, we offer FITC‑conjugated detection tools and can assist in securing complementary antibodies, recombinant proteins, and technical consultation to advance your development pipeline.