FITC Rabbit Anti-Human/Monkey CD8a Monoclonal Antibody for FC - P01732

Core Product Specifications and Parameters

Biological Background: CD8a Function and Localization

• CD8A encodes the T-cell surface glycoprotein CD8 alpha chain, also known as T8/Leu-2, a key marker of cytotoxic T lymphocytes.
• As an integral membrane glycoprotein, CD8a functions as a coreceptor for MHC class I molecules, binding peptide antigens derived from cytosolic proteins and facilitating T-cell receptor (TCR) recognition.
• Upon TCR-MHC class I engagement, CD8a recruits the Src kinase LCK to the TCR-CD3 complex, initiating intracellular signaling cascades that promote T-cell activation, lymphokine production, and cytotoxic responses.
• In cytotoxic T-lymphocytes (CTLs), CD8a-mediated signaling is critical for the elimination of infected cells and tumor cells, underpinning adaptive cellular immunity.
• In natural killer (NK) cells, CD8A homodimers provide a survival mechanism that supports conjugation with and lysis of multiple target cells.
• CD8a homodimers also contribute to the differentiation and maintenance of memory CD8+ T-cells, ensuring long-term immune surveillance.
• Subcellularly, CD8a localizes primarily to the cell membrane but can also be secreted, with tissue expression observed on thymus-derived T-cells (α/β heterodimer or α/α homodimer), NK cells, memory T-cells, intraepithelial lymphocytes, monocytes, and dendritic cells.
• Post-translational modifications include N-linked glycosylation, disulfide bond formation, and palmitoylation, as highlighted by UniProt keywords.

Experimental Guidance and Technical Tips

• This FITC-conjugated antibody targets the extracellular domain (aa 22-182) of human CD8A; verify performance in your specific cell model.
• For flow cytometry, titrate the antibody to determine the optimal working concentration for your sample type (e.g., PBMCs, whole blood).
• Use appropriate live/dead staining and isotype controls to ensure specific detection of CD8a+ populations.
• Validate cross-reactivity in cynomolgus monkey samples, as indicated by manufacturer data.
• Fixing/permeabilizing cells may affect epitope recognition; optimize staining protocols accordingly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio positions itself as your one-stop sourcing partner for IVD raw materials, delivering validated antibodies and ancillary reagents from concept to clinic. Whether you need bulk monoclonal antibodies for diagnostic kit development or specialized reagents for immune cell profiling, our supply network streamlines procurement of CD8a and other immune-oncology targets. Contact our team to discuss custom sourcing, technical support, and scalable solutions tailored to your project.