FITC Rabbit anti-Human/Monkey CD45 Monoclonal Antibody for FC - P08575

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45, also known as PTPRC, is a receptor-type tyrosine-protein phosphatase that serves as the leukocyte common antigen (L-CA or T200). It acts as a positive regulator of T-cell activation through the antigen receptor upon binding to DPP4, and is required for effective T-cell coactivation. Related references: PMID:35767951
• The intracellular region harbors two phosphatase domains: the first domain has enzymatic activity, while the second modulates substrate specificity. CD45 dephosphorylates key signaling intermediates such as SKAP1, FYN, and LYN, thereby fine-tuning T-cell receptor signaling thresholds.
• Alternative splicing generates multiple isoforms (1–8) with variable extracellular domains. In thymocytes, isoforms 1, 2, 3, 5, and 7 are detected, whereas isoforms 4, 6, and 8 are absent, indicating tightly regulated isoform expression during T-cell development.
• Subcellular localization includes the cell membrane, membrane rafts, and the immunological synapse, where CD45 dynamically interacts with antigen-presenting cells to modulate T-cell activation and limit autoreactivity.
• As a heavily glycosylated and phosphorylated protein, CD45 exhibits an apparent molecular weight range of 131–147 kDa on SDS-PAGE, reflecting extensive post-translational modifications that influence its function and isoform diversity.
• CD45 also participates in host-pathogen interactions: it acts as a receptor for human cytomegalovirus protein UL11, leading to impaired T-cell proliferation upon viral engagement.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and instrument configuration.
• Use human PBMCs or cynomolgus lymphocyte suspensions as positive controls; CD45 is highly expressed on all leukocyte subsets, providing a robust gating marker.
• Incorporate this FITC conjugate into multicolor panels alongside markers for T cells, B cells, and myeloid cells to enable comprehensive immunophenotyping.
• Ensure the cytometer is equipped with a 488 nm laser and appropriate filters for FITC detection.
• Validate staining with an appropriate isotype control and include Fc receptor blocking to minimize non-specific binding.
• Note that the epitope maps to the extracellular domain (aa 26–577), which is shared across multiple CD45 isoforms, supporting broad leukocyte recognition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw materials, offering validated antibody pairs, monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target procurement to accelerate your CD45-based assay development. Our comprehensive sourcing capabilities cover the full pipeline—from concept to clinic—empowering diagnostic manufacturers, laboratories, and research institutes with reliable reagents for leukocyte marker detection and immune cell studies.