FITC Rabbit anti-Human CD99 Monoclonal Antibody for FC - P14209

Core Product Specifications and Parameters

Biological Background: CD99 Function and Localization

• CD99 (also known as MIC2, E2 antigen, or T-cell surface glycoprotein E2) is a type I transmembrane glycoprotein expressed on the surface of various hematopoietic and non-hematopoietic cells.
• It mediates T-cell adhesion and facilitates spontaneous rosette formation with erythrocytes, a hallmark of T-cell biology.
• A key function of CD99 is in leukocyte extravasation: it specifically regulates the late step of diapedesis through the endothelial basement membrane, acting independently of PECAM1.
• CD99 is localized to the plasma membrane and features a single transmembrane helix, with both intracellular and extracellular domains.
• Alternative splicing generates multiple CD99 isoforms, which may exhibit distinct tissue distributions and functions.
• The protein is subject to post-translational modifications, including N-glycosylation and phosphorylation, which are important for its adhesive properties.
• The predicted molecular weight of the CD99 protein backbone is approximately 18.8 kDa, with the mature glycoprotein migrating at around 19 kDa in SDS-PAGE.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to FITC, reducing background and streamlining staining protocols for flow cytometry.
• The immunogen covers the extracellular domain, suggesting recognition of extracellular epitopes; consider its use for live-cell surface staining.
• Titrate the antibody to determine the optimal concentration for your specific cell type and include appropriate isotype controls and Fc blocking.
• CD99 expression may serve as a marker for T-cell subsets and in studies of leukocyte migration; validate performance in your experimental system.
• The antibody has been validated for human samples; cross-reactivity with other species is not reported and should be tested if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your IVD development with a comprehensive portfolio of raw materials, including this anti-CD99 monoclonal antibody for immunophenotyping and leukocyte research applications. Beyond antibodies, we offer a full range of ancillary reagents, bulk supply options, and sourcing services for rare or custom targets, enabling seamless integration into your diagnostic workflow.