FITC Rabbit anti-Human CD7 Monoclonal Antibody for Flow Cytometry - P09564

Core Product Specifications and Parameters

Biological Background: CD7 Function and Localization

• CD7, also known as GP40, Tp40, and Leu-9, is a single-pass type I transmembrane glycoprotein encoded by the CD7 gene. It belongs to the immunoglobulin superfamily and plays a critical role in adaptive immunity.
• CD7 functions as a costimulatory receptor on T-cells, enhancing their activation upon binding to its ligand K12/SECTM1. This interaction promotes the production of cytokines such as interleukin-2 (IL-2), a key growth factor for T-cell proliferation and effector function. Related references: PMID:10652336 PMID:1709867
• On natural killer (NK) cells and their precursors, CD7 engagement triggers a robust induction of interferon-gamma (IFN-γ), a pivotal cytokine for innate and adaptive immune responses. Related references: PMID:7506726
• The protein is anchored in the plasma membrane via a single transmembrane helix, with its N-terminal extracellular region containing immunoglobulin-like domains responsible for ligand recognition. The short cytoplasmic tail mediates downstream signaling.
• Post-translational modifications of CD7 include disulfide bond formation essential for structural stability and palmitoylation (S-palmitate) that likely modulates membrane association and signal transduction.
• CD7 is a well-established lineage marker routinely used in flow cytometry-based immunophenotyping to identify and characterize T-cell and NK-cell subsets in human samples.

Experimental Guidance and Technical Tips

• Titrate the FITC-conjugated antibody to determine the optimal concentration for your specific cell type and assay conditions; typical starting concentrations for flow cytometry range from 0.5–5 µg per 1×10⁶ cells.
• Always include an appropriate isotype control (FITC-conjugated rabbit IgG with irrelevant specificity) to set gates and compensate for non-specific binding.
• Consider using a viability dye to exclude dead cells, which may exhibit increased autofluorescence and non-specific antibody binding.
• Due to the FITC fluorophore, protect stained samples from prolonged light exposure to minimize photobleaching, and acquire data promptly on the flow cytometer.
• Validate staining in relevant sample systems, such as human peripheral blood mononuclear cells (PBMCs) or established T-cell lines (e.g., Jurkat), to confirm expected reactivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutions with streamlined access to high-quality IVD raw materials, including this anti-human CD7 monoclonal antibody optimized for flow cytometry. We support your T-cell immunophenotyping and diagnostic assay development with a broad portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and targeted sourcing for rare antigens. Partner with us to enhance your workflow from discovery to clinical application.