FITC Rabbit anti-Human CD44 Monoclonal Antibody for FC - P16070

Core Product Specifications and Parameters

Biological Background: CD44 Function and Localization

• Protein identity and isoforms: CD44 antigen (also known as CDw44, Epican, ECMR-III, HUTCH-I, PGP-1, Hermes antigen, hyaluronate receptor) is a transmembrane glycoprotein with multiple isoforms generated by alternative splicing. Isoform 10 (epithelial isoform) is prominently expressed in carcinomas.
• Core function in cell adhesion and migration: Functions as a cell-surface receptor for hyaluronan and other extracellular matrix components, mediating cell-cell and cell-matrix interactions critical for migration and tissue homeostasis. Related references: PMID:16541107, PMID:19703720, PMID:22726066
• Role in immune cell homing and inflammation: Participates in T-lymphocyte activation, recirculation, and homing, as well as hematopoiesis and inflammatory responses. Related references: PMID:7528188
• Signal transduction platform: Upon ligand engagement, its cytoplasmic domain assembles protein complexes containing receptor kinases and membrane proteases, integrating microenvironmental cues. Related references: PMID:18757307, PMID:23589287
• Cytoskeletal reorganization and NK cell activation: Coordinates Rho GTPase (RAC1, RHOA) and phospholipase C pathways to drive actin reorganization. Interaction with LGALS9 triggers LCK/ERK/MAPK signaling in NK cells. Related references: PMID:15123640, PMID:37006235
• Microbial infection: Promotes foot-and-mouth disease virus internalization via macropinocytosis.
• Subcellular localization and tissue expression: Localizes to the cell membrane, microvilli, and secreted fractions. Detected in fibroblasts, urine, and placenta; isoform 10 is epithelial-specific and highly expressed in carcinomas.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 20–178) corresponds to the extracellular hyaluronan–binding domain. This region is highly conserved and likely allows the antibody to recognize native CD44 in flow cytometry.
• As a FITC conjugate, this antibody is ready for direct immunofluorescence staining. Titrate the antibody to determine the optimal working concentration for your specific cell type and experimental conditions.
• For flow cytometry on human samples, consider using Fc receptor blocking and appropriate viability dyes to minimize nonspecific binding.
• The antibody’s reactivity with other species has not been characterized; users should verify cross-reactivity independently if applied to non-human systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing solution for IVD raw materials, including validated monoclonal antibodies like this FITC-conjugated anti-CD44 antibody for flow cytometry applications. Our portfolio supports immunology and oncology research with bulk ancillary reagents, optimized antibody pairs, and access to rare targets. We collaborate with diagnostic manufacturers and labs to streamline development from concept to clinic.