FITC Rabbit anti-Human CD2 Monoclonal Antibody for FC - P06729

Core Product Specifications and Parameters

Biological Background: CD2 Function and Localization

• CD2 is a T-cell surface antigen, also known as T11, SRBC (sheep red blood cell receptor), and LFA-2. It mediates adhesion between T-cells and other cell types via interaction with CD58 (LFA-3) and CD48/BCM1.
• The protein is a member of the immunoglobulin superfamily, containing immunoglobulin-like domains, and plays key roles in cell adhesion and T-cell signaling.
• CD2 is implicated in T-cell triggering, with its cytoplasmic domain involved in signaling functions.
• Expressed on the surface of T-cells and natural killer cells (at protein level), CD2 localizes to the cell membrane as a single-pass type I membrane protein.
• Post-translational modifications include glycosylation and the presence of disulfide bonds, as indicated in UniProt keywords: Glycoprotein, Disulfide bond.
• The extracellular region interacts with LFA-3 on antigen-presenting cells, facilitating immune synapse formation and T-cell activation.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to FITC, making it suitable for direct immunofluorescence flow cytometry without a secondary antibody. Optimize the antibody dilution for your cell type and experimental setup.
• Use appropriate controls (isotype control, unstained cells, positive controls such as T-cell lines or peripheral blood lymphocytes) to validate staining specificity.
• The immunogen corresponds to the extracellular domain (amino acids 25-209), so the antibody is expected to recognize native surface CD2 on live cells. For intracellular staining, carefully optimize fixation and permeabilization conditions.
• Consider titrating the antibody to determine the optimal staining concentration for your flow cytometry panel, especially when multiplexing with other fluorophores.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This CD2 antibody supports immunophenotyping and immune function research, and CamelBio can assist with custom sourcing for specialized diagnostic needs, helping manufacturers streamline their supply chain from concept to clinic.