FITC Anti-Mouse CD3e Monoclonal Antibody - CD3e

Core Product Specifications and Parameters

Experimental Guidance and Technical Tips

• Flow Cytometry (FC) Application: This FITC-conjugated antibody is suitable for direct immunofluorescence staining of mouse cells. Optimize the working concentration by titration on a known positive cell population (e.g., mouse splenocytes) to minimize background and achieve optimal signal-to-noise ratio.
• Isotype Control: Use an Armenian hamster IgG isotype control conjugated to FITC to assess nonspecific binding. Ensure the isotype control matches the fluorophore and host species.
• Cross Reactivity: This antibody is specific for mouse CD3e. Reaction with other species has not been tested; consider validating cross-reactivity if used in non-mouse systems.
• Sample Preparation: For surface staining, standard protocols for live/dead cell discrimination and Fc receptor blocking (e.g., anti-CD16/CD32) are recommended to reduce nonspecific binding.
• Storage and Handling: Ship on ice. Upon receipt, store at 2–8°C protected from light. Do not freeze; repeated freezing may deteriorate the fluorophore.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material sourcing partner, CamelBio provides comprehensive access to high-quality antibodies like this FITC Anti-Mouse CD3e mAb for immunology research and diagnostic assay development. Beyond individual reagents, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to streamline your workflow. Our global supply network also supports custom requirements, including rare target raw-material sourcing, empowering your projects from concept to clinic.