FIP200 Rabbit pAb - Q8TDY2

Core Product Specifications and Parameters

Biological Background: RB1-inducible coiled-coil protein 1 Function and Localization

• RB1-inducible coiled-coil protein 1 (RB1CC1), also known as FIP200, is a 183 kDa scaffold protein characterized by coiled-coil domains.
• It serves as a key autophagy regulator, orchestrating autophagosome biogenesis through direct interaction with ATG16L1. Related references: PMID:21775823 PMID:23392225
• In innate immunity, FIP200 participates in xenophagy by targeting intracellular bacteria such as Salmonella.
• It suppresses FAK and PYK2 kinase activities, affecting cell migration and survival signals. Related references: PMID:10769033 PMID:12221124
• Acts as a transcription factor that upregulates RB1 expression, linking it to cell cycle control and tumor suppression. Related references: PMID:12095676 PMID:14533007
• It is required for myoblast differentiation and contributes to hematopoietic and neuronal development. Related references: PMID:12163359
• The protein shuttles between the nucleus and cytoplasm, localizes to preautophagosomal membranes and lysosomes, and is subject to alternative splicing and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1280–1400, a region predicted to be exposed; consider this when designing blocking peptides or confirming epitope specificity.
• For Western blot, a primary antibody dilution of 1:500–1:2000 may serve as a starting point, but optimal working concentration should be experimentally determined.
• For IHC-P, antigen retrieval using citrate buffer (pH 6.0) is often effective for large coiled-coil proteins; validate in appropriate control tissues.
• Verify cross-reactivity with mouse and rat samples, as sequence homology in the immunogen region may influence detection sensitivity.
• As a polyclonal antibody, it may recognize multiple isoforms or post-translationally modified forms; confirm specificity via siRNA knockdown or knockout cell lines.

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