FHL1 Rabbit pAb for WB and ELISA - Q13642

Core Product Specifications and Parameters

Biological Background: FHL1 Function and Localization

• FHL1 (Four and a half LIM domains protein 1), also known as SLIM or SLIMMER, is a member of the FHL family characterized by an N-terminal half LIM domain followed by four complete LIM domains.
• FHL1 is implicated in muscle development and hypertrophy, playing a regulatory role in skeletal muscle growth and differentiation.
• It localizes to the cytoplasm, nucleus, and cytosol, suggesting dynamic shuttling that may link cytoskeletal signaling to transcriptional regulation.
• Isoform 1 is predominantly expressed in skeletal muscle, with lower levels in heart, placenta, and other tissues; isoforms 2 and 3 show more restricted patterns including brain and testis.
• The LIM domains coordinate zinc ions, enabling protein-protein interactions critical for sarcomere assembly and signal transduction.
• Post-translational modifications include acetylation and ubiquitination, which may modulate protein stability and function.
• Mutations in FHL1 are associated with Emery-Dreifuss muscular dystrophy and other myopathies, highlighting its essential role in muscle integrity.
• Alternative splicing produces multiple isoforms, each with distinct tissue expression and presumably specialized functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 27-296 of human FHL1, covering most of the protein; due to the polyclonal nature, the antibody may recognize multiple epitopes and isoforms.
• For Western blot, expect a band around 36 kDa; validate specificity using appropriate positive and negative controls, such as skeletal muscle lysate.
• Cross-reactivity with mouse is confirmed; researchers should verify antibody performance in their specific mouse tissue or cell line.
• ELISA applications should be optimized with the relevant antigen; the polyclonal antibody can serve as a capture or detection reagent after pairing validation.
• Not tested for IHC or ICC; use in these applications would require additional validation.
• Shipping on ice bag ensures stability; aliquot and store at -20°C upon arrival.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated polyclonal and monoclonal antibodies like this anti-FHL1 antibody, supporting muscle disease research and diagnostic development. As a one-stop sourcing partner, we provide optimized antibody pairs, bulk ancillary reagents, and rare target raw-material procurement to accelerate your project from concept to clinic. Contact us to streamline your supply chain and ensure reliable, high-quality materials.