FGFR2 Rabbit Polyclonal Antibody for IHC-P and ELISA - P21802

Core Product Specifications and Parameters

Biological Background: FGFR2 Function and Localization

• FGFR2 is a tyrosine-protein kinase receptor that binds fibroblast growth factors, regulating cell proliferation, differentiation, migration, and apoptosis.
• It is crucial for embryonic development, including limb bud formation, lung morphogenesis, and osteogenesis, and is required for normal skeleton development.
• FGFR2 exists in multiple isoforms due to alternative splicing, including the keratinocyte growth factor receptor (KGFR) and the bacterially expressed kinase (BEK) variant.
• Upon ligand binding, FGFR2 activates downstream signaling pathways such as PLCG1, RAS-MAPK, and AKT1, mediated through phosphorylation of adaptor proteins like FRS2.
• Subcellularly, FGFR2 is located at the cell membrane, Golgi apparatus, and cytoplasmic vesicles; it can also be secreted.
• Key post-translational modifications include glycosylation, ubiquitination, and phosphorylation, which regulate its activity and turnover.
• Mutations in FGFR2 are associated with craniosynostosis, ectodermal dysplasia, and various skeletal disorders, highlighting its clinical significance.

Experimental Guidance and Technical Tips

• For IHC-P, consider optimizing antigen retrieval conditions, as the antibody targets an N-terminal epitope (amino acids 1-100). Validate in the relevant tissue types from human, mouse, or rat.
• In ELISA, the synthetic peptide immunogen may serve as a suitable positive control for assay calibration and specificity testing.
• The predicted molecular weight is ~92kDa; however, multiple splice isoforms exist. When used in Western blotting, validate the detected bands against known isoform patterns in your sample system.
• Given the high sequence conservation across human, mouse, and rat for the immunogen region, cross-reactivity is expected, but confirm with appropriate controls in your specific application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with high-quality IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. For FGFR2-related projects in oncology, developmental biology, or skeletal disorder research, we offer one-stop sourcing from monoclonal antibodies to bulk peptide antigens to support assay development and biomarker studies. Our supply network ensures consistent access to rare and specialized raw materials, accelerating your path from concept to clinic.