FGF23 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9GZV9

Core Product Specifications and Parameters

Biological Background: FGF23 Function and Localization

• FGF23 (Fibroblast growth factor 23), also known as Phosphatonin or ADHR, is a secreted protein that functions as a key regulator of phosphate homeostasis. Related references: PMID:11062477
• Inhibits renal tubular phosphate reabsorption by downregulating the sodium-phosphate cotransporter SLC34A1. Related references: PMID:11409890
• Regulates vitamin D metabolism by modulating 1,25-dihydroxyvitamin D3 synthesis. Related references: PMID:15040831
• Negatively controls osteoblast differentiation and bone matrix mineralization. Related references: PMID:18282132
• Functions in the presence of klotho (KL) to up-regulate EGR1 expression and acts on the parathyroid gland to suppress PTH secretion (By similarity).
• Predominantly expressed in osteogenic cells, osteocytes, and bone-lining cells during active bone remodeling; localized to the secreted compartment.
• Post-translational modifications include glycosylation and disulfide bond formation; classified as a growth factor with disease-associated variants.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 25-251 of human FGF23, covering the mature protein and ensuring reactivity with endogenous and recombinant forms.
• In Western blotting under reducing conditions, FGF23 typically migrates at approximately 28 kDa; however, post-translational modifications may produce additional bands.
• For IF/ICC, confirm appropriate signal localization (secreted/extracellular) and consider using blocking agents to reduce background.
• This polyclonal antibody cross-reacts with mouse and rat FGF23, expanding its utility for comparative studies across species.
• ELISA can be developed using this antibody paired with a matched detection antibody; optimize coating and detection conditions for each system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents, to support diagnostic manufacturers from research to commercialization. For FGF23, a critical marker in phosphate metabolism and metabolic bone diseases, we offer high-quality polyclonal antibodies backed by stringent quality control. Our one-stop sourcing model streamlines procurement of rare targets and bulk reagents, accelerating your assay development timeline.