FGF2 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P09038

Core Product Specifications and Parameters

Biological Background: FGF2 Function and Localization

• FGF2 (Fibroblast growth factor 2), also known as basic FGF (bFGF) or HBGF-2, is a heparin-binding growth factor encoded by the FGF2 gene.
• FGF2 acts as a ligand for FGFR1, FGFR2, FGFR3, and FGFR4, and also binds integrin ITGAV:ITGB3, thereby playing a crucial role in regulating cell survival, division, differentiation, and migration. Related references: PMID:8663044 PMID:28302677
• It functions as a potent mitogen in vitro, stimulating cell proliferation. Related references: PMID:1721615 PMID:3732516 PMID:3964259
• FGF2 induces angiogenesis, a process essential for tissue repair and pathological conditions such as tumor growth. Related references: PMID:23469107 PMID:28302677
• Mediates ERK1/2 phosphorylation, promoting retinal lens fiber differentiation. Related references: PMID:29501879
• Subcellularly, FGF2 is found secreted and in the nucleus. Tissue expression includes granulosa and cumulus cells, with overexpression observed in hepatocellular carcinoma cells compared to non-cancerous liver tissue.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within amino acids 100-200 of human FGF2, suggesting this antibody may recognize a linear epitope. It is suitable for applications involving denatured proteins, such as Western blotting under reducing conditions.
• For Western blotting, a principal band at approximately 31 kDa is expected. Consider that post-translational modifications or alternative initiation isoforms could result in additional bands.
• When performing immunohistochemistry or immunofluorescence, remember that FGF2 is secreted and may also localize to the nucleus; include appropriate permeabilization and blocking steps in your protocol.
• The antibody has been tested in ELISA, indicating its utility in quantitative detection of native or recombinant FGF2.
• As a rabbit monoclonal antibody, it offers high specificity and minimal lot-to-lot variation. Validate the antibody in the relevant sample system before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and academic institutes with a streamlined, one-stop sourcing solution for IVD raw materials, supporting you from concept to clinic. For FGF2-focused research, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to accelerate your angiogenesis, oncology, or developmental biology studies. Contact us to discuss your custom raw-material sourcing requirements and technical support needs.