FGD4 Rabbit pAb (Polyclonal Antibody) for WB, ELISA - Q96M96

Core Product Specifications and Parameters

Biological Background: FGD4 Function and Localization

• FGD4, also known as FYVE, RhoGEF and PH domain-containing protein 4, actin filament-binding protein frabin, or FGD1-related F-actin-binding protein, is encoded by the FGD4 gene and has multiple isoforms due to alternative splicing.
• FGD4 functions as a guanine nucleotide exchange factor (GEF) that specifically activates CDC42, a Rho-family GTPase, by catalyzing the exchange of GDP for GTP, thereby regulating actin cytoskeleton organization and cell shape.
• The protein localizes to the cytoplasm, associates with the actin cytoskeleton, and is also found at cell projections such as filopodia, where it influences actin dynamics.
• FGD4 is expressed in various tissues including brain, cerebellum, peripheral nerve, skeletal muscle, heart, uterus, placenta, and testis, indicating broad yet specific physiological roles.
• Post-translational modifications include phosphorylation, and the protein contains functional domains such as FYVE-type zinc finger, reflecting its metal-binding and phosphoprotein regulatory properties.
• Mutations in FGD4 are associated with Charcot-Marie-Tooth disease type 4H (CMT4H), a neurodegenerative disorder, highlighting its critical role in peripheral nerve function.

Experimental Guidance and Technical Tips

• For Western blot (WB), anticipate a band around 87 kDa consistent with the predicted molecular weight; validate using appropriate positive controls derived from FGD4-expressing tissues (e.g., brain, peripheral nerve, or skeletal muscle).
• Since the immunogen covers amino acids 99–198, consider the possibility of detecting multiple isoforms; cross-check with alternative detection methods if isoform-specific resolution is needed.
• In ELISA applications, standardize coating concentrations and antibody dilutions using recombinant FGD4 protein or cell lysates to establish optimal signal-to-noise ratios.
• Because FGD4 is involved in cytoskeletal regulation, fixation and permeabilization protocols may affect epitope accessibility; test different conditions when employing immunofluorescence.
• Confirm cross-reactivity in mouse and rat samples using appropriate lysates, as sequence homology may lead to detection of the rodent orthologs.

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