FGB Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P02675

Core Product Specifications and Parameters

Biological Background: Fibrinogen Beta Chain (FGB) Function and Localization

• FGB encodes the fibrinogen beta chain, a secreted glycoprotein that, together with fibrinogen alpha (FGA) and gamma (FGG), constitutes the fibrinogen hexamer, a major coagulation factor in blood.
• Upon cleavage by thrombin, fibrinogen beta chain monomers polymerize with other chains to form insoluble fibrin, the structural basis of blood clots.
• Fibrin plays critical roles in hemostasis, wound healing (stabilizing lesions and guiding cell migration), and pregnancy maintenance.
• Studies indicate that while fibrinogen enhances SELP expression in activated platelets, it is not absolutely required for thrombus formation in vivo.
• Fibrin deposition at infection sites protects against IFNG-mediated hemorrhage and may support antibacterial immune responses via innate and T-cell pathways.
• The protein is secreted and detected in blood plasma; it contains structural features like a coiled-coil domain and post-translational modifications including signal peptide, disulfide bonds, glycosylation, and pyrrolidone carboxylic acid.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 111-222, a region that may be conserved across human, mouse, and rat FGB, supporting the observed cross-reactivity; validate in the relevant species sample system.
• For western blot, the expected band is approximately 56 kDa; consider using plasma or tissue lysates and optimizing reducing conditions.
• In IF/ICC, co-staining with other fibrinogen chains can help evaluate fibrin matrix formation; confirm staining specificity with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering a wide portfolio of validated antibodies, including polyclonal and matched pairs, for coagulation and hemostasis research. We support diagnostic developers and labs with bulk reagents and rare target sourcing for the fibrinogen beta chain and related biomarkers. Our supply chain expertise helps streamline your transition from concept to clinic.