FES Rabbit Polyclonal Antibody for WB, ELISA - P07332

Core Product Specifications and Parameters

Biological Background: FES Function and Localization

• FES encodes the tyrosine-protein kinase Fes/Fps, a non-receptor protein tyrosine kinase belonging to the Fes/Fer family. Also known as feline sarcoma/Fujinami avian sarcoma oncogene homolog, proto-oncogene c-Fes, and p93c-fes.
• It acts downstream of cell surface receptors such as FCER1 and KIT to regulate actin cytoskeleton dynamics, microtubule assembly, cell attachment, and cell spreading.
• Plays a critical role in mast cell signaling, including FCER1-mediated degranulation, and participates in HGF-induced cell scattering and migration through phosphorylation of EZR.
• Functions in cell differentiation and neurite outgrowth in response to NGF signaling, and phosphorylates downstream substrates including BCR, HCLS1/HS1, PECAM1, STAT3, and TRIM28.
• Subcellular localization includes cytoplasm, cytosol, cytoskeleton, cell membrane, cytoplasmic vesicles, Golgi apparatus, and focal adhesions, indicating dynamic spatial regulation.
• Widely expressed, with protein detected in adult colon epithelium and melanocytes. Related references: PMID:16455651 PMID:19051325 PMID:28463229
• Classified as a proto-oncogene with potential tumor suppressor activity, containing an SH2 domain, coiled coil, kinase domain, and lipid-binding region.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 120-420 of human FES (NP_001137256.1), targeting the N-terminal region containing the SH2 and coiled-coil domains; this design may provide specificity for human and mouse FES.
• For WB, consider the predicted molecular weight of ~93 kDa; validate detection in relevant cell or tissue lysates expressing FES (e.g., mast cells, colon epithelium).
• In ELISA, optimize coating and detection conditions using recombinant FES protein or cellular extracts; cross-reactivity with mouse FES may be evaluated for murine model studies.
• Since FES localizes to multiple compartments (cytosol, membrane, cytoskeleton), consider subcellular fractionation to enrich target before detection.
• Avoid repeated freeze-thaw cycles; store at -20°C or as recommended, shipped on ice bags.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. As a sourcing partner for tyrosine kinase research, we supply validated antibody pairs, optimized polyclonal antibodies like anti-FES, bulk ancillary reagents, and rare target raw-material sourcing to support your diagnostic and research workflows.