FCRL3 Rabbit Polyclonal Antibody for WB and ELISA - Q96P31

Core Product Specifications and Parameters

Biological Background: FCRL3 Function and Localization

• FCRL3 (Fc receptor-like protein 3), also known as FcRH3, IRTA3, MAIA, and CD307c, is a type I transmembrane glycoprotein with immunomodulatory roles in both adaptive immunity and fertilization.
• Promotes TLR9-induced B-cell proliferation, activation, and survival while inhibiting antibody production and suppressing plasma cell differentiation; enhances NF-kB and MAPK signaling pathways. Related references: PMID:23857366
• Exerts inhibitory potential on B-cell receptor (BCR)-mediated signaling, likely through association with SH2 domain-containing phosphatases, thereby reducing tyrosine phosphorylation, calcium mobilization, and activation-induced cell death. Related references: PMID:19843936
• Regulatory T-cells expressing FCRL3 display a memory phenotype, are hyporesponsive to antigenic stimulation in the presence of IL-2, and have diminished capacity to suppress effector T-cell proliferation. Related references: PMID:19494275, PMID:20190142
• Serves as a human-specific oolemma epitope essential for sperm-egg adhesion and fusion; interacts with the IZUMO1-IZUMO1R/JUNO complex and acts as the IZUMO1 receptor during fertilization, replacing JUNO. Related references: PMID:36070373
• Localizes predominantly to the cell membrane and microvillus membrane, consistent with its roles in immune synapse regulation and gamete interaction.
• Primarily expressed in secondary lymphoid tissues on mature B-cell subsets, with lower levels on transitional B cells and increased surface expression on memory and innate-like B cells; also detected in NK cells, unfertilized oocytes, and certain thymically derived regulatory T cells. Found in spleen, lymph node, peripheral blood, thymus, bone marrow, kidney, salivary gland, adrenal gland, and uterus.
• Contains immunoglobulin domains, a signal peptide, a transmembrane helix, and undergoes glycosylation, phosphorylation, and alternative splicing, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen (aa 600–720 of human FCRL3) lies within the cytoplasmic tail, so the antibody primarily detects intracellular epitopes; for immunofluorescence, permeabilization may be necessary, but confirm protocol compatibility.
• For Western blotting, a band at approximately 81 kDa is expected; however, glycosylation and isoform variants may cause slight shifts. Validate with positive controls such as B-cell lines (e.g., Raji, Daudi) or lymphoid tissue lysates.
• In ELISA, consider using recombinant FCRL3 protein or cell-based assays; optimize coating concentration and detection range empirically.
• Cross-reactivity with mouse and rat is reported, but due to sequence divergence in the target region, verify specificity and signal intensity in those species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials and technical services for diagnostic manufacturers and researchers focusing on immunology, B-cell biology, and reproductive science. Our offerings include validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to support your entire development pipeline—from concept to clinic. With this FCRL3 antibody, we help accelerate assay development for studies on adaptive immunity, immune regulation, and fertilization.