FCHSD1 Rabbit pAb - Q86WN1

Core Product Specifications and Parameters

Biological Background: FCHSD1 Function and Localization

• FCHSD1 (F-BAR and double SH3 domains protein 1), also known as Protein nervous wreck 2 (NWK2), is a scaffolding protein containing an N-terminal F-BAR domain, two SH3 domains, and a coiled-coil region.
• The protein promotes actin polymerization by linking the membrane-deforming F-BAR domain to the actin regulatory machinery via its interaction with SNX9 and WASL (N-WASP).
• Alternative splicing generates multiple isoforms, which may modulate its interaction network and subcellular distribution.
• FCHSD1 localizes primarily to the cytoplasm and is enriched at the perikaryon. It also associates with cell projections and cytoplasmic vesicles, consistent with a role in membrane trafficking and cytoskeletal reorganization.
• The SH3 domains mediate protein-protein interactions, while the F-BAR domain binds lipids and induces membrane curvature, coupling membrane dynamics to actin assembly.
• Phosphorylation of FCHSD1 may regulate its activity or localization, as indicated by its identification as a phosphoprotein in large-scale proteomic studies.
• No tissue-specific expression pattern has been documented, but as a nervous wreck homolog, it may be relevant in neuronal contexts where actin dynamics are critical.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 100–400, which includes portions of the F-BAR and SH3 domains; therefore, the polyclonal antibody may recognize both full-length and truncated isoforms.
• For Western blot, a band near 77 kDa is expected; however, due to alternative splicing, additional bands may appear. Validate by using control lysates from human, mouse, or rat cells.
• For IHC-P, antigen retrieval conditions should be optimized. Test on positive control tissues such as brain or tissues with known FCHSD1 expression, considering the perikaryal localization.
• In ELISA, the recombinant immunogen can serve as a positive coating antigen to assess antibody reactivity and titer.
• Cross-reactivity with mouse and rat indicates potential for use in rodent models; verify specificity with knockout or knockdown samples if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, contract research organizations, and academic laboratories with a comprehensive portfolio of IVD-grade antibodies and ancillary reagents. This FCHSD1 rabbit polyclonal antibody supports research into actin dynamics, membrane trafficking, and neurobiology. As your sourcing partner, we also supply validated antibody pairs, optimized monoclonal antibodies, bulk reagents, and rare-target raw materials, streamlining your procurement from discovery to clinical application.