FBLN1 Rabbit Polyclonal Antibody for WB, ELISA - P23142

Core Product Specifications and Parameters

Biological Background: Fibulin-1 (FBLN1) Function and Localization

• Fibulin-1, encoded by the FBLN1 gene, is a secreted glycoprotein that incorporates into fibronectin-containing matrix fibers and contributes to extracellular matrix (ECM) architecture, particularly basement membranes.
• It may play roles in cell adhesion, migration along protein fibers, and developmental processes, suggesting involvement in tissue organization.
• Fibulin-1 exhibits tumor suppressor activity and is implicated in cellular transformation and tumor invasion; it also modulates the neurotrophic activities of amyloid precursor protein (APP).
• The protein can bind fibrinogen and incorporate into clots, indicating a potential role in haemostasis and thrombosis.
• Multiple isoforms exist: isoforms A and B are placenta-specific, while isoforms C and D are widely expressed in various tissues and cultured cells.
• Fibulin-1 is located in the extracellular space and matrix, and it contains EGF-like domains, calcium-binding sites, and disulfide bonds, as well as being subject to alternative splicing and glycosylation.
• The protein has a molecular weight of approximately 77 kDa and is identified by multiple aliases, including FBLN and FIBL1.

Experimental Guidance and Technical Tips

• Since the immunogen targets a specific region (amino acids 570–670), consider verifying isoform specificity if your experiment requires distinguishing between FBLN1 variants.
• For Western blot, the predicted molecular weight is ~77 kDa; validate the band in your sample system using appropriate controls and consider possible post-translational modifications.
• In ELISA applications, optimize coating conditions with recombinant FBLN1 or relevant lysates, and confirm sensitivity with known standards.
• Cross-reactivity with mouse and rat suggests conserved epitopes; perform sequence alignment to assess suitability for non-human models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, including validated polyclonal antibodies like this anti-FBLN1 antibody, to support research in extracellular matrix biology and cancer diagnostics. As your one-stop sourcing partner, we offer optimized antibody pairs, bulk ancillary reagents, and efficient sourcing of rare-target raw materials, helping you advance seamlessly from concept to clinic.