FASTKD5 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q7L8L6

Core Product Specifications and Parameters

Biological Background: FASTKD5 Function and Localization

• FASTKD5 is a mitochondrial protein that functions as an endoribonuclease essential for processing non-canonical mitochondrial pre-mRNAs, such as MT-CO1, MT-CO3, and MT-CYB, generating products with distinct 3'-end modifications. Related references: PMID:25683715, PMID:40499538, PMID:40637235
• It is localized to the mitochondrial matrix and nucleoid.
• Tissue expression is broad, with highest levels in heart, smooth muscle, liver, and thyroid.
• Post-translational modifications include acetylation and phosphorylation.
• It belongs to the FAST kinase domain-containing protein family and is associated with primary mitochondrial disease when mutated.

Experimental Guidance and Technical Tips

• The immunogen (aa 495-764) spans a conserved region, supporting cross-reactivity with mouse and rat FASTKD5; validate in relevant sample systems.
• For Western blot, expect a band at approximately 87 kDa; use mitochondrial-enriched lysates (e.g., heart or muscle) for optimal detection.
• In IF/ICC, consider co-staining with mitochondrial markers to confirm subcellular localization.
• For ELISA, titrate the antibody and include appropriate controls to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides FASTKD5 antibodies as part of our comprehensive IVD raw-material portfolio, supporting mitochondrial disease research and assay development. Our one-stop sourcing bridge connects diagnostic manufacturers with validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, streamlining procurement from concept to clinic.