Fast Myosin Skeletal Heavy chain Rabbit mAb - P12882

Core Product Specifications and Parameters

Biological Background: Myosin-1 Function and Localization

• Myosin-1 (also known as myosin heavy chain 1, MyHC-2x, MyHC-IIx/d) is encoded by the MYH1 gene and belongs to the class II myosin heavy chain family. It is primarily expressed in adult skeletal muscle fast-twitch fibers.
• Required for normal hearing; contributes to cochlear amplification by positively regulating prestin (SLC26A5) activity and outer hair cell electromotility.
• Possesses actin-binding and ATPase motor domains, enabling force generation along actin filaments; an essential component of thick filaments in myofibrils.
• Localizes to the cytoplasm and myofibrils, specifically the A-band of sarcomeres in skeletal muscle.
• Predicted to undergo phosphorylation and methylation, which may modulate its activity or protein interactions.
• Contains calmodulin-binding motifs and coiled-coil domains for dimerization, characteristic of myosin heavy chains.

Experimental Guidance and Technical Tips

• For Western blotting, a band of approximately 223 kDa is expected; use appropriate large-protein transfer conditions and high-molecular-weight markers.
• In IHC-P, consider optimizing antigen retrieval protocols and validate staining in the tissue of interest.
• The immunogen targets the N-terminal region (aa 1–100); verify isoform specificity if multiple MYH1 variants are present in your sample.
• Cross-reactivity with mouse and rat enables use in rodent models; confirm performance with individual species samples and appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw material sourcing partner offering validated antibodies, bulk reagents, and customized solutions for assays targeting skeletal muscle and auditory research. Whether you need this MYH1 monoclonal antibody, matched antibody pairs, or ancillary reagents, we streamline procurement from concept to clinic. Our technical team provides tailored support to accelerate your diagnostic development projects.