FasLG Rabbit pAb - P48023

Core Product Specifications and Parameters

Biological Background: FasLG Function and Localization

• FasLG (CD95L, TNFSF6) is a type II transmembrane protein belonging to the TNF ligand superfamily.
• It is the canonical ligand for the death receptor FAS (CD95), and this interaction triggers the extrinsic apoptotic pathway crucial for immune cell-mediated cytotoxicity (PubMed:26334989, PubMed:7528780, PubMed:9228058, PubMed:9427603). Related references: PMID:26334989 PMID:7528780 PMID:9228058 PMID:9427603
• FasLG also binds to the decoy receptor TNFRSF6B/DcR3, which can inhibit FAS-dependent apoptosis (PubMed:27806260). Related references: PMID:27806260
• Plays an essential role in activation-induced cell death (AICD) of T-cells, contributing to the termination of immune responses and peripheral tolerance (By similarity).
• Ligation of FAS can additionally activate NF-kappa-B, leading to non-apoptotic signals involved in cell survival and inflammatory responses (By similarity).
• The protein exists in membrane-anchored and soluble forms; the cleaved soluble form may diffuse to exert paracrine effects, while the cytoplasmic domain has been reported to have transcription inhibitory activity.
• Subcellularly, FasLG localizes primarily to the plasma membrane, but also to cytoplasmic vesicles, lysosomes, and the nucleus, and is secreted via exosomes.
• Post-translational modifications include disulfide bond formation, N-linked glycosylation, and ubiquitination, which affect trafficking and protein stability (Keywords: Glycoprotein, Disulfide bond, Ubl conjugation).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the central region (aa 101-200); consider verifying reactivity with the soluble shed form if required.
• For Western blotting, a band around 31 kDa is expected under reducing conditions; cleavage products may be detected at lower molecular weights.
• In IHC-P, antigen retrieval may be necessary; validate staining with appropriate positive and negative controls.
• For ELISA, the antibody can be used as a capture or detection reagent after proper pairing validation; test with recombinant protein or native samples.
• Cross-reactivity with mouse is predicted; confirm specificity in mouse tissue or cell models if used for non-human applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to providing diagnostic manufacturers and research institutes with a comprehensive range of IVD raw materials. For FasLG-related projects, we offer validated polyclonal and monoclonal antibodies, recombinant proteins, and ancillary reagents to support immunoassay development. Our global supply network ensures rapid access to rare-target raw materials, backed by technical and consulting services from concept to clinic.