FANCD2 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - Q9BXW9

Core Product Specifications and Parameters

Biological Background: FANCD2 Function and Localization

• FANCD2 (Fanconi anemia group D2 protein) is essential for maintaining chromosomal stability and promotes accurate homolog pairing during meiosis. Related references: PMID:11239453 PMID:14517836
• Plays a critical role in DNA double-strand break repair via homologous recombination and single-strand annealing pathways. Related references: PMID:15671039 PMID:15650050 PMID:30335751
• Participates in S phase and G2 phase checkpoint activation in response to DNA damage. Related references: PMID:15377654
• Prevents breakage and loss of missegregating chromatin during cell division, particularly after replication stress, and is required for BLM recruitment to replicative stress-induced structures. Related references: PMID:15454491 PMID:15661754 PMID:19465921
• Promotes loading of BRCA2/FANCD1 onto damaged chromatin, facilitating downstream repair. Related references: PMID:12239151 PMID:12086603
• Localizes predominantly to the nucleus and undergoes monoubiquitination in response to DNA damage.
• Highly expressed in germinal center cells of lymphoid tissues, proliferating squamous epithelia, cytotrophoblasts, exocrine pancreas, and maturing spermatocytes in testis.
• Keywords: Alternative splicing, Cell cycle, Disease variant, DNA damage, DNA repair, Fanconi anemia, Isopeptide bond, Nucleus, Phosphoprotein, Ubl conjugation.

Experimental Guidance and Technical Tips

• For Western blot, a major band at approximately 164 kDa is expected; monoubiquitinated FANCD2 may appear as a slower-migrating species upon DNA damage induction. Consider using DNA-damaging agents (e.g., mitomycin C or hydroxyurea) in relevant cell lines to assess activation.
• In immunofluorescence/ICC, nuclear staining is anticipated. Optimize fixation and permeabilization conditions to preserve epitope accessibility.
• For immunoprecipitation, this rabbit polyclonal antibody may enrich FANCD2 and its interacting partners. Validate IP efficiency in the specific cellular model under study.
• Cross-reactivity with mouse and rat FANCD2 is reported; sequence alignment of the immunogen region suggests conserved epitopes, but independent validation in these species is recommended.
• ELISA application may require direct coating or capture format; titrate the antibody to determine optimal working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined sourcing solution for IVD raw materials, including this FANCD2 polyclonal antibody for DNA repair and Fanconi anemia research. Beyond individual antibodies, we supply validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and custom sourcing for rare target raw materials—supporting your workflow from concept to clinic.