FAF1 Rabbit Polyclonal Antibody (WB/ELISA) - Q9UNN5

Core Product Specifications and Parameters

Biological Background: FAS-associated factor 1 Function and Localization

• FAF1 (FAS-associated factor 1) is a ubiquitin-binding protein that interacts with polyubiquitinated substrates through its UBX domain. Related references: PMID:19722279
• Plays a critical role in DNA replication by targeting the replication licensing factor CDT1 for ubiquitin-dependent degradation, ensuring proper replication fork progression. Related references: PMID:26842564
• Potentiates FAS-induced apoptosis, although it cannot initiate the apoptotic signal on its own.
• The gene undergoes alternative splicing, giving rise to multiple protein isoforms.
• Localizes predominantly to the nucleus, consistent with its role in DNA replication and apoptosis regulation.
• Is a phosphoprotein, indicating that its activity may be modulated by phosphorylation.
• Tissue expression is highest in testis, followed by skeletal muscle and heart, with lower levels in prostate, thymus, ovary, small intestine, and colon; it is not detected in peripheral blood leukocytes.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-280). For Western blot, consider running a positive control lysate (e.g., HeLa or testis tissue) and validate the observed band near 74 kDa.
• For ELISA, the polyclonal nature of the antibody may provide robust signal but should be titrated against the specific coating antigen.
• Cross-reactivity with mouse and rat has been indicated; validate detection in the relevant sample systems to confirm species-specific performance.
• Since FAF1 undergoes alternative splicing, multiple bands may appear; verify isoform specificity with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, supporting diagnostic manufacturers and research labs with validated antibody pairs, bulk ancillary reagents, and custom monoclonal/polyclonal antibody development. For projects focused on apoptosis, DNA replication, or ubiquitin-pathway targets like FAF1, our platform streamlines procurement from concept to clinic, ensuring timely supply of rare-target raw materials.