FADD Rabbit PolymAb® Monoclonal Antibody for WB, IP, IHC-P, ELISA - Q61160 / Q13158

Core Product Specifications and Parameters

Biological Background: FADD Function and Localization

• FADD (FAS-associated death domain protein), also known as Mort1, is an adapter molecule essential for death receptor-mediated apoptosis.
• It recruits initiator caspases such as caspase-8 (CASP8) to the death-inducing signaling complex (DISC) upon activation of FAS/CD95 or TNFR1, triggering apoptotic cascades.
• Beyond apoptosis, FADD participates in innate immunity by positively regulating interferon signaling during antiviral responses.
• The protein is encoded by the FADD gene, with human Gene ID 8772 and mouse Gene ID 14082; it is highly conserved across species.
• Subcellularly, FADD localizes predominantly to the cytoplasm where it interacts with death receptors.
• Key post-translational modifications include phosphorylation, which modulates its function and interactions.
• UniProt keywords: 3D-structure, Apoptosis, Cytoplasm, Immunity, Innate immunity, Phosphoprotein, Reference proteome.

Experimental Guidance and Technical Tips

• For Western blotting (WB), a band of approximately 23 kDa is expected; run appropriate positive and negative controls.
• In immunoprecipitation (IP), use optimized lysis buffers to preserve protein interactions involving death domains.
• For immunohistochemistry (IHC-P), consider antigen retrieval protocols and validate staining specificity with relevant tissue controls.
• When using in ELISA, test both capture and detection formats to determine optimal performance.
• As a recombinant rabbit monoclonal antibody (PolymAb®), expect high batch-to-batch consistency; nevertheless, validate each new lot in your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner for diagnostic manufacturers and research laboratories. This FADD antibody is part of our expanding catalog that supports apoptosis, innate immunity, and oncology research. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents—backed by dedicated technical consultation—to streamline your assay development from concept to clinic.