F4/80 Rabbit mAb for IF, IHC-P, ELISA - Q61549

Core Product Specifications and Parameters

Biological Background: F4/80 Function and Localization

• F4/80, encoded by the Adgre1 (Emr1) gene, is an adhesion G protein-coupled receptor (GPCR) belonging to the EGF-TM7 subfamily.
• The full-length protein is a cell surface glycoprotein with a molecular weight of approximately 102 kDa, containing multiple EGF-like domains and a seven-transmembrane domain.
• F4/80 functions as an orphan receptor thought to mediate cell adhesion and cell-cell interactions, particularly within the immune system.
• It is specifically expressed on the surface of macrophages, with highest levels on mature tissue-resident macrophages, but is absent from macrophages located in T-cell areas of lymph nodes and spleen.
• Low-level expression is also detected on blood monocytes, making F4/80 a well-established pan-macrophage marker in mice.
• The receptor may contribute to the development and function of regulatory T cells (Tregs), linking innate and adaptive immunity.
• Post-translational modifications include glycosylation and disulfide bond formation, which are typical for EGF-like domain-containing proteins and important for structural stability.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant fragment (aa 367–645) from the extracellular region of mouse F4/80, targeting a sequence with potentially less glycosylation interference; verify that this epitope is exposed in your fixed samples.
• For immunofluorescence (IF) on paraffin-embedded sections (IF-P), consider antigen retrieval with citrate buffer (pH 6.0) or EDTA (pH 9.0) and optimize primary antibody incubation conditions based on the tissue type.
• In immunohistochemistry on paraffin sections (IHC-P), validate the antibody in a known F4/80-positive control (e.g., mouse liver Kupffer cells or splenic red pulp macrophages) and include an isotype control to assess background staining.
• When performing ELISA, use the antibody as a capture or detection reagent against mouse F4/80 recombinant protein and cross-verify reactivity with rat F4/80 if the assay is intended for rat samples.
• As a rabbit monoclonal antibody, it offers batch-to-batch consistency; however, confirm specific reactivity in your experimental system, especially if using knockout or knockdown models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this F4/80 rabbit monoclonal antibody as part of a comprehensive portfolio of IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes in developing robust immunological assays. In addition to validated antibodies, we supply bulk ancillary reagents and can source rare-target raw materials, ensuring seamless integration of critical components like validated antibody pairs, optimized monoclonal/polyclonal antibodies, and custom reagents for your workflow. As your one-stop partner from concept to clinic, CamelBio simplifies the procurement of high-quality immunological tools for macrophage and immune research.