EXTL3 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O43909

Core Product Specifications and Parameters

Biological Background: EXTL3 Function and Localization

• EXTL3 (Exostosin-like 3), also known as EXT-related protein 1, multiple exostosis-like protein 3, or putative tumor suppressor protein EXTL3, is a member of the exostosin-like gene family, which includes EXTL1, EXTL2, and EXTL3.
• Acts as a key glycosyltransferase initiating heparan sulfate (HS) biosynthesis by transferring the first N-acetyl-alpha-D-glucosamine (GlcNAc) residue to the core protein linker, and may also participate in HS chain elongation; lacks glucuronyl transferase II activity. Related references: PMID:11390981, PMID:35676258
• Essential for skeletal development and hematopoiesis through the formation of HS proteoglycans (HSPGs), and regulates postnatal pancreatic islet maturation and insulin secretion (by similarity). Related references: PMID:11390981, PMID:22727489, PMID:28132690
• Acts as a receptor for REG3A, REG3B, and REG3G, triggering downstream PI3K-AKT or RAS-RAF-MEK-ERK signaling pathways; mediates REG3A-induced keratinocyte proliferation and differentiation, inhibition of skin inflammation, and is necessary for REG3A/REG3G-dependent glucose tolerance and microglial responses to prevent endotoxic death. Related references: PMID:22727489, PMID:27830702, PMID:34099862, PMID:19158046
• Subcellularly localizes to the endoplasmic reticulum membrane, Golgi apparatus, cell membrane, and nucleus.
• Ubiquitously expressed, with notable presence in keratinocytes and pancreas.
• According to UniProt, EXTL3 is a glycoprotein and phosphoprotein, disease-associated variants are linked to dwarfism and intellectual disability, and enzymatic activity requires manganese.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 52–220) targets an N-terminal region, likely detecting both glycosyltransferase and receptor functionalities of EXTL3; consider validating in systems with relevant biological contexts.
• For western blotting, expect a band near 105 kDa; membrane-enriched or nuclear protein extracts may enhance detection due to its subcellular distribution.
• For IHC-P, antigen retrieval is typically required; tissues such as pancreas and skin, where EXTL3 is highly expressed, can serve as positive controls.
• ELISA protocols may be optimized using the recombinant immunogen or purified EXTL3 protein to establish assay conditions; cross-reactivity with mouse and rat homologs allows flexibility in model selection.
• As a polyclonal antibody, batch-to-batch consistency should be confirmed; consider including appropriate controls to account for potential off-target recognition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a seamless one-stop sourcing experience for IVD raw materials, offering this EXTL3 polyclonal antibody as part of a broader portfolio that includes validated antibody pairs, bulk ancillary reagents, and sourcing support for rare targets. Whether you are developing assays for heparan sulfate biosynthesis or REG3-mediated signaling, our supply chain expertise and technical guidance help accelerate your transition from concept to clinic. Partner with CamelBio to secure high-quality, research-grade antibodies and comprehensive procurement solutions.