EXOSC4 Rabbit Polyclonal Antibody for WB, ELISA - Q9NPD3

Core Product Specifications and Parameters

Biological Background: EXOSC4 Function and Localization

• EXOSC4 (also known as RRP41, SKI6, or p12A) is a non-catalytic component of the RNA exosome complex, a multi-subunit machinery with 3′→5′ exoribonuclease activity.
• In the nucleus, the exosome contributes to the maturation of stable RNA species (rRNA, snRNA, snoRNA) and eliminates processing by-products, pervasive transcripts (PROMPTs), and defective mRNAs.
• The exosome may participate in immunoglobulin class switch recombination and somatic hypermutation by targeting AICDA deaminase activity to transcribed DNA substrates.
• In the cytoplasm, the exosome promotes general mRNA turnover, including the degradation of ARE-containing mRNAs and aberrant transcripts in RNA surveillance.
• EXOSC4 specifically binds to AU-rich elements (AREs) in the 3′ untranslated regions of mRNAs, targeting them for decay.
• Subcellular localization includes the cytoplasm, nucleolus, nucleoplasm, and nucleus, consistent with its roles in nuclear and cytoplasmic RNA metabolism.
• Post-translational modifications of EXOSC4 have been identified, including acetylation, and the protein has been validated by direct protein sequencing and proteomics.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1–245, which constitutes the full-length human EXOSC4 protein; therefore, the polyclonal antibody may recognize both native and denatured conformations in Western blot.
• For ELISA applications, consider direct antigen coating or capture formats, and optimize antibody concentrations using a titration series.
• Cross-reactivity with rat allows use in rat-derived samples; validation in the specific tissue or cell line of interest is recommended.
• Endogenous EXOSC4 migrates at ~26 kDa; loading controls and molecular weight markers near this size should be included in WB experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This EXOSC4 antibody supports investigations into RNA processing and degradation pathways, including exosome-related disorders. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to meet your procurement needs.