EXOC3 Rabbit Polyclonal Antibody for WB, ELISA - O60645

Core Product Specifications and Parameters

Biological Background: EXOC3 Function and Localization

• EXOC3, also known as Sec6, is a component of the exocyst complex, which plays a critical role in tethering secretory vesicles to the plasma membrane prior to fusion.
• The exocyst complex is essential for exocytosis, directing vesicles to specific docking sites and ensuring accurate delivery of cargo.
• EXOC3 is localized in the cytoplasm, particularly in the perinuclear region, and associates with the Golgi apparatus, reflecting its involvement in post-Golgi trafficking.
• In neuronal cells, EXOC3 localizes to growth cones and neuron projections, contributing to polarized secretion and membrane expansion.
• The protein is also found at the midbody during cytokinesis, suggesting a role in membrane trafficking during cell division.
• Tissue-specific expression has been detected in the epididymis, where exocyst function may be involved in secretion processes.
• Post-translational modifications, including acetylation, have been identified by proteomic analyses, potentially regulating its function.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to a C-terminal region (amino acids 396-745) of human EXOC3, which may provide specificity for the full-length protein across species.
• This polyclonal antibody is recommended for Western blot (WB) and ELISA applications, based on supplier validation. For other applications, optimization and validation in the relevant sample system are advised.
• Given the reported cross-reactivity with human, mouse, and rat, consider using appropriate species-specific controls to confirm binding specificity in each model.
• When performing WB, a band around 86 kDa is expected. Ensure protein extraction and loading conditions are optimized for your sample type.
• For ELISA, determine optimal coating and detection conditions empirically, as performance may vary with antigen presentation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing access to validated IVD raw materials, including this EXOC3 antibody suitable for exocyst complex and exocytosis studies. Whether you require optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, or rare target sourcing, our supply network is designed to streamline procurement from concept to clinic. Contact us for technical consultation and tailored material solutions.