EWSR1 Rabbit Polyclonal Antibody for IF/ICC, ELISA - Q01844

Core Product Specifications and Parameters

Biological Background: EWSR1 Function and Localization

• EWSR1 specifically binds to single-stranded RNA containing the consensus sequence 5'-AGGUAA-3'.
  Related references:
  PMID:21256132
• It may function as a transcriptional repressor.
  Related references:
  PMID:10767297
• EWS-fusion-proteins, arising from chromosomal rearrangements, are implicated in tumorigenesis and may contribute to aberrant transcriptional activation of target genes.
• The protein localizes to the nucleus, cytoplasm, and cell membrane.
• EWSR1 is ubiquitously expressed in human tissues.
• Post-translational modifications include acetylation, methylation, and phosphorylation.
• It contains a zinc-finger domain and a calmodulin-binding motif, suggesting roles in metal ion coordination and calcium signaling.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal 300 amino acids of human EWSR1, a region present in both wild-type and most EWS-fusion proteins; consider validating detection specificity in your target sample type.
• For IF/ICC, optimize fixation and permeabilization conditions to ensure proper localization detection; include appropriate positive and negative controls.
• For ELISA, this polyclonal antibody may serve as a detection antibody; pairing with a capture antibody should be validated using recombinant protein or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw material partner, CamelBio supports diagnostic manufacturers and research labs with a comprehensive portfolio, including validated antibody pairs and optimized polyclonal antibodies like this anti-EWSR1 pAb for cancer research applications. We streamline the sourcing of bulk ancillary reagents and rare target raw materials, ensuring reliable supply from concept to clinic.