EWSR1 Rabbit Monoclonal Antibody for WB, IHC-P - Q01844

Core Product Specifications and Parameters

Biological Background: EWSR1 Function and Localization

• EWSR1 encodes EWS RNA-binding protein 1, also known as EWS oncogene or Ewing sarcoma breakpoint region 1 protein. It is a member of the TET family of RNA-binding proteins.
• The protein binds single-stranded RNA recognizing the consensus sequence 5'-AGGUAA-3' (PubMed:21256132). Related references: PMID:21256132
• EWSR1 functions as a transcriptional repressor, modulating gene expression (PubMed:10767297). Related references: PMID:10767297
• Chromosomal translocations involving EWSR1 generate fusion proteins (e.g., EWS-FLI1) that act as aberrant transcription factors, driving tumorigenesis in Ewing sarcoma and other cancers.
• The protein localizes predominantly to the nucleus, but can also be found in the cytoplasm and at the cell membrane.
• EWSR1 is ubiquitously expressed across tissues and contains zinc-finger domains, calmodulin-binding motifs, and multiple post-translational modifications including acetylation, methylation, and phosphorylation.
• Alternative splicing yields multiple isoforms, contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen region (aa 1-189) is at the N-terminus of EWSR1, which is retained in many fusion proteins. This antibody may therefore detect both wild-type EWSR1 and certain EWSR1 fusion oncoproteins.
• For Western blot (WB), the predicted molecular weight is 68 kDa; consider the use of appropriate controls and validate in relevant cell lines.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), nuclear staining is expected; include negative controls and optimize antigen retrieval conditions.
• Cross-reactivity with mouse and rat species has been reported; confirm specificity in your own experimental systems.
• As a rabbit monoclonal antibody, it offers high affinity and low batch-to-batch variability, suitable for quantitative applications like ELISA and IP.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw-material supplier, CamelBio supports diagnostic manufacturers and researchers with validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For targets like EWSR1 in oncology diagnostics, we offer one-stop sourcing solutions that streamline assay development from concept to clinic. Contact us to explore customized procurement options for your next project.