ETV4 Rabbit Polyclonal Antibody for WB, ELISA - P43268

Core Product Specifications and Parameters

Biological Background: ETV4 Function and Localization

• ETV4 (ETS translocation variant 4) is a member of the ETS family of transcription factors, also known as PEA3, E1A-F, or PEAS3. Multiple isoforms are generated through alternative splicing.
• It acts as a transcriptional activator, binding to specific DNA sequences to regulate gene expression. In the nervous system, it controls motor neuron positioning and terminal arborization (By similarity), while in the skin it influences keratinocyte differentiation (PubMed:31552090). Related references: PMID:19307308 PMID:31552090
• ETV4 is predominantly localized in the nucleus, consistent with its function as a transcription factor.
• The protein is subject to post-translational modifications, including phosphorylation and ubiquitin-like modifications (isopeptide bond), which may affect its stability and activity.
• During adenovirus infection, ETV4 binds to the viral E1A enhancer and activates transcription via a core sequence 5'-[AC]GGA[AT]GT-3', highlighting its role in viral gene expression.
• ETV4 expression is detected in keratinocytes, suggesting a role in epidermal biology.

Experimental Guidance and Technical Tips

• When performing Western blotting, a band around 54 kDa is anticipated; however, due to post-translational modifications, the observed molecular weight may shift slightly. Validate in your experimental system.
• Given the polyclonal nature, this antibody may recognize multiple ETV4 isoforms. Check for additional bands, especially in samples with known alternative splicing events.
• For ELISA, titrate the antibody and use appropriate positive controls such as recombinant ETV4 protein or nuclear extracts from relevant cell lines.
• If studying ETV4 function in motor neurons or keratinocytes, consider using human, mouse, or rat samples to leverage known cross-reactivity.
• Since the immunogen corresponds to a central region (aa 40-246), the antibody likely recognizes multiple domains; however, confirm specificity with knockdown or knockout models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw-material procurement, providing comprehensive access to antibodies like this ETV4 polyclonal antibody. We support research and diagnostic development in transcription, neurogenesis, and infectious disease fields. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, as well as bulk ancillary reagents and custom sourcing for rare targets.