ETS1 Rabbit pAb - P14921

Core Product Specifications and Parameters

Biological Background: ETS1 Function and Localization

• ETS1 functions as a transcription factor, playing a key role in gene regulation (PubMed:10698492, PubMed:11909962). Related references: PMID:10698492, PMID:11909962
• It directly controls the expression of cytokine and chemokine genes in diverse cellular contexts (PubMed:20378371). Related references: PMID:20378371
• ETS1 may regulate the differentiation, survival, and proliferation of lymphoid cells (PubMed:20378371). Related references: PMID:20378371
• It potentially regulates angiogenesis by modulating genes involved in endothelial cell migration and invasion (PubMed:15247905, PubMed:15592518). Related references: PMID:15247905, PMID:15592518
• The isoform c-ETS-1A acts as a dominant-negative regulator of ETS1 function.
• ETS1 localizes to both the nucleus and the cytoplasm.
• It is highly expressed in lymphoid cells; isoform expression varies with tissue type in adults, and is absent in brain and kidney.
• Post-translational modifications of ETS1 include phosphorylation, acetylation, and ubiquitination, reflecting its dynamic regulation.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to the N-terminal region (amino acids 1–260) of human ETS1, which is shared across canonical isoforms. Researchers should verify isoform specificity in their experimental system.
• For Western blot (WB), a band around 50 kDa is expected; however, due to potential post‑translational modifications or isoform variation, additional bands may be observed. Validate with appropriate controls.
• For immunohistochemistry (IHC‑P) and immunofluorescence (IF/ICC), consider optimizing antigen retrieval and antibody dilution using positive and negative tissue or cell samples.
• This antibody has been tested in ELISA; it may be suitable for quantitative assays, but users should confirm linearity and sensitivity with recombinant proteins or known standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for IVD raw materials, offering a seamless bridge from concept to clinic. This high-quality anti‑ETS1 rabbit polyclonal antibody supports research into transcription regulation, immune function, and angiogenesis. In addition to validated antibodies, we provide optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and expert sourcing for rare targets to streamline your diagnostic development needs.