ETNK2 Rabbit Polyclonal Antibody for WB, ELISA - Q9NVF9

Core Product Specifications and Parameters

Biological Background: ETNK2 Function and Localization

• ETNK2 encodes ethanolamine kinase 2, a key enzyme in the CDP-ethanolamine pathway for phosphatidylethanolamine biosynthesis.
• The protein is highly specific for ethanolamine phosphorylation and does not exhibit choline kinase activity (By similarity).
• Alternative splicing produces multiple transcript variants, which may result in different isoforms.
• ETNK2 is expressed in kidney, liver, ovary, testis, and prostate, indicating a tissue-specific role in phospholipid metabolism.
• As a kinase, it binds ATP and nucleotide, and is involved in lipid biosynthesis and metabolism.
• It lacks choline kinase activity, distinguishing it from closely related enzymes.

Experimental Guidance and Technical Tips

• The immunogen comprises the full-length recombinant protein (amino acids 1-386), suggesting the antibody may recognize multiple isoforms and native conformations.
• For Western blotting, consider using lysates from tissues with high expression such as kidney, liver, or testis; the predicted molecular weight is 45 kDa.
• In ELISA, the polyclonal nature may provide signal amplification; validate in the relevant sample matrix.
• Cross-reactivity with other species has not been tested; verify if used beyond human samples.

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